Related Experiment Video

Updated: Jan 4, 2026

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
12:21

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

Published on: November 30, 2013

15.3K

LNCRNA CDKN2B-AS1 regulates mesangial cell proliferation and extracellular matrix accumulation via miR-424-5p/HMGA2

Yang Li1, Lin-Lin Zheng2, Deng-Gao Huang2

  • 1Department of Nephropathy, Central South University Xiangya School of Medicine Affiliated Haikou Hospital, Haikou, Hainan, China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|November 11, 2019
PubMed
Abstract

Insights

Long noncoding RNA CDKN2B-AS1 promotes diabetic nephropathy by increasing cell proliferation and extracellular matrix accumulation. Silencing CDKN2B-AS1 may offer a therapeutic strategy for diabetic nephropathy treatment.

Area of Science:

  • Nephrology
  • Molecular Biology
  • Genetics

Background:

  • Diabetic nephropathy (DN) is a complication of diabetes with abnormal expression of long noncoding RNA CDKN2B-AS1.
  • The precise mechanism of CDKN2B-AS1 in DN progression requires further investigation.

Purpose of the Study:

  • To elucidate the role of CDKN2B-AS1 in diabetic nephropathy.
  • To investigate the molecular mechanism involving microRNA-424-5p and HMGA2.

Main Methods:

  • Quantitative real-time PCR and western blot were used to detect expression levels of CDKN2B-AS1, miR-424-5p, and HMGA2.
  • Dual-luciferase reporter and RNA immunoprecipitation assays confirmed target associations.
  • Cell proliferation, ECM accumulation, and PI3K/AKT signaling were assessed in high glucose-treated human glomerular mesangial cells (HGMC).

Main Results:

  • CDKN2B-AS1 was upregulated, while miR-424-5p was downregulated in DN patients and HGMC cells exposed to high glucose.
  • CDKN2B-AS1 acted as a sponge for miR-424-5p, and HMGA2 was a target of miR-424-5p.
  • Silencing CDKN2B-AS1 or overexpressing miR-424-5p repressed cell proliferation, ECM accumulation, and the PI3K/AKT pathway, partly by targeting HMGA2.

Conclusions:

  • Knockdown of CDKN2B-AS1 inhibits HGMC proliferation, ECM accumulation, and PI3K/AKT signaling.
  • This effect is mediated by the upregulation of miR-424-5p and downregulation of HMGA2.
  • CDKN2B-AS1 plays a crucial role in DN pathogenesis via the miR-424-5p/HMGA2 axis.
Keywords:
CDKN2B-AS1Diabetic nephropathyHMGA2Human glomerular mesangial cellsmiR-424-5p

More Related Videos

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells11:42

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

9.8K
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

8.0K

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.0K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.7K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.6K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.3K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
7.6K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
10.3K