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Progestogen profiling in plasma during the estrous cycle in cattle using an LC-MS based approach.

A K Hankele1, K Rehm2, J Berard3

  • 1ETH Zurich, Animal Physiology, Institute of Agricultural Sciences, Universitaetstr. 2, 8092, Zurich, Switzerland.

Theriogenology
|November 12, 2019
PubMed
Summary

Progesterone metabolites, including allopregnanolone and pregnanolone, are present in bovine plasma during the estrous cycle. Their levels change with progesterone, particularly around luteolysis.

Keywords:
BovineEstrous cycleLC-MSProgestogen

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Area of Science:

  • Reproductive Endocrinology
  • Neurosteroidology
  • Veterinary Science

Background:

  • Progesterone (P4) is crucial for mammalian reproduction, with active metabolites influencing reproductive functions.
  • Metabolites of progesterone can interact with progesterone receptors and GABAA receptors, impacting physiological processes.

Purpose of the Study:

  • To investigate the presence and profiles of reduced progesterone metabolites in bovine plasma throughout the estrous cycle.
  • To compare the temporal dynamics of these metabolites with progesterone (P4) levels, especially during luteolysis.

Main Methods:

  • Plasma samples from cattle during the estrous cycle were analyzed using liquid chromatography-mass spectrometry (LC-MS).
  • Quantification of progesterone (P4) and its various reduced metabolites was performed.

Main Results:

  • Reduced progestogens were detected in bovine plasma, exhibiting profiles that mirrored progesterone (P4) during the estrous cycle.
  • Progestogen levels lagged behind the decline of P4 during luteolysis.
  • The most abundant metabolites were P4, followed by allopregnanolone, pregnanolone, epipregnanolone, and 20β-DHP.

Conclusions:

  • Progesterone metabolites are present in bovine plasma and their concentrations correlate with P4 levels during the estrous cycle.
  • Further research is needed to fully elucidate the role of these metabolites around the critical period of luteolysis.