Related Experiment Video
Updated: Jan 4, 2026
Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum
Published on: October 26, 2019
Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum
Shahin Shabanipour1, Azadeh Dalvand1, Xiaodan Jiao1
1Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba; The Children's Hospital Research Institute of Manitoba (CHRIM), Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba.
Abstract:
The use of primary cell cultures has become one of the major tools to study the nervous system in vitro. The ultimate goal of using this simplified model system is to provide a controlled microenvironment and maintain the high survival rate and the natural features of dissociated neuronal and nonneuronal cells as much as possible under in vitro conditions. In this article, we demonstrate a method of isolating primary neurons from the developing mouse cerebellum, placing them in an in vitro environment, establishing their growth, and monitoring their viability and differentiation for several weeks. This method is applicable to embryonic neurons dissociated from cerebellum between embryonic days 12-18.
