Renewable electrochemical sensor for PARP-1 activity detection based on host-guest recognition

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Biochemistry

Background:

• Poly (ADP-ribose) polymerase-1 (PARP-1) is an emerging biomarker of significant clinical interest.
• Accurate detection of PARP-1 activity is crucial for various diagnostic applications.
• Existing detection methods may face limitations in sensitivity, selectivity, or reusability.

Purpose of the Study:

• To develop a novel, renewable electrochemical sensor for sensitive and selective detection of PARP-1 activity.
• To utilize host-guest recognition for enhanced sensor performance.
• To establish a platform for efficient and reusable PARP-1 detection.

Main Methods:

• Modification of an electrode surface with mono-(6-Mercapto-6-deoxy)-beta-Cyclodextrin (SH-β-CD) for host-guest recognition.
• Immobilization of trans-azobenzene labeled dsDNA (Abz-dsDNA) onto the SH-β-CD modified electrode.
• Detection of PARP-1 activity via the generation of phosphate ions and subsequent reaction with molybdate to form a detectable current signal.
• Electrode surface regeneration using UV irradiation to cleave the Abz-dsDNA via trans-cis isomerization of azobenzene.

Main Results:

• The electrochemical sensor demonstrated a linear detection range for PARP-1 activity from 0.01 U to 1.0 U.
• A low detection limit of 0.008 U was achieved, indicating high sensitivity.
• The sensor effectively avoided non-specific adsorption, leading to improved detection accuracy.
• UV-induced regeneration allowed for the effective recycling of the electrode surface.

Conclusions:

• The developed renewable electrochemical sensor exhibits high sensitivity and selectivity for PARP-1 activity detection.
• The host-guest recognition mechanism and renewable feature offer advantages over existing methods.
• This sensor shows significant potential as a tool for clinical diagnostics and biochemical research.