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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Mass Spectrometers01:16

Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
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Related Experiment Video

Updated: Jan 4, 2026

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
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[Mass spectrometry-applications in pathology].

K Schwamborn1

  • 1Institut für Allgemeine Pathologie und Pathologische Anatomie, Technische Universität München, Trogerstr. 18, 81675, München, Deutschland. kschwamborn@tum.de.

Der Pathologe
|November 13, 2019
PubMed
Summary
This summary is machine-generated.

Proteomic technologies, particularly mass spectrometry, offer faster and more accurate diagnostic confirmation using less sample material. Imaging mass spectrometry enables intact tissue analysis, overcoming limitations of traditional methods for various cancers.

Keywords:
Hodgkin diseaseMass spectrometry imagingMatrix-assisted laser desorption-ionization mass spectrometryProstate cancerProteomics

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Medical Diagnostics

Background:

  • Current diagnostics require extensive work-up, often on limited samples, including genomic analyses.
  • Protein-level analysis offers a dynamic view of biological changes, making proteomic technologies a promising solution.
  • Integrating proteomic approaches can enhance diagnostic capabilities.

Purpose of the Study:

  • To evaluate diverse proteomic techniques for analyzing body fluids and tissue samples.
  • To assess the diagnostic implementation potential of these proteomic methods.
  • To compare proteomic approaches with existing diagnostic strategies.

Main Methods:

  • Utilized mass spectrometry-based proteomic techniques.
  • Applied methods to analyze serum and tissue samples from various patient groups.
  • Investigated imaging mass spectrometry for intact tissue analysis.

Main Results:

  • Mass spectrometry effectively differentiates patient groups across diseases.
  • Classical proteomics suits serum analysis (e.g., bladder cancer, hepatitis C).
  • Imaging mass spectrometry analyzes intact tissues, preserving histological information, with demonstrated utility in prostate cancer, Hodgkin's lymphoma, cervical cancer, and adenocarcinomas.

Conclusions:

  • Mass spectrometry-based proteomic technologies provide high sensitivity and specificity for diagnostic confirmation.
  • These methods offer faster results with reduced sample requirements compared to routine diagnostics.
  • Proteomics presents a valuable, accurate, and efficient alternative for disease diagnosis.