Related Experiment Video

Updated: Jan 4, 2026

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis
13:47

Laser Capture Microdissection of Highly Pure Trabecular Meshwork from Mouse Eyes for Gene Expression Analysis

Published on: June 3, 2018

9.8K

Role of matrix metalloproteinase-9 gene polymorphisms in glaucoma: A hospital-based study in Chinese patients

Fengqiong Zhao1, Zongshan Fan1, Xuewen Huang1

  • 1Department of Ophthalmology, Nanchong Central Hospital, Nanchong, China.

Journal of Clinical Laboratory Analysis
|November 13, 2019
PubMed
Abstract

Insights

Common variants of matrix metalloproteinase-9 (MMP-9) are linked to glaucoma development in the Chinese population. Specific MMP-9 gene mutations correlate with elevated protein levels, increasing glaucoma risk.

Area of Science:

  • Ophthalmology
  • Genetics
  • Biochemistry

Background:

  • Glaucoma is a leading cause of irreversible blindness globally.
  • Matrix metalloproteinase-9 (MMP-9) plays a role in extracellular matrix remodeling and has been implicated in glaucoma.
  • Previous studies suggest associations between MMP-9 variants and glaucoma in various populations.

Purpose of the Study:

  • To investigate the association of common MMP-9 gene variants with different clinical subtypes of glaucoma in the Chinese population.
  • To determine if specific MMP-9 polymorphisms correlate with plasma MMP-9 levels.

Main Methods:

  • Genotyping of five common MMP-9 single nucleotide polymorphisms (SNPs) using PCR-RFLP in 396 glaucoma patients (212 PACG, 184 POAG) and 329 healthy controls.
  • Quantification of plasma MMP-9 levels using ELISA.

Main Results:

  • The rs2250889 polymorphism (allele G) and rs17576 variant were significantly more frequent in both primary angle-closure glaucoma (PACG) and primary open-angle glaucoma (POAG) cases compared to controls.
  • The rs3918242 polymorphism was more prevalent in POAG cases than in controls.
  • Significant associations were found between MMP-9 polymorphisms (rs2250889, rs3918242, rs17576) and plasma MMP-9 levels.

Conclusions:

  • Specific MMP-9 gene variants are associated with an increased risk of developing glaucoma in the Chinese population.
  • These MMP-9 variants are linked to elevated plasma MMP-9 levels, suggesting a mechanism for glaucoma predisposition.
Keywords:
angle closuregenetic polymorphismsglaucomamatrix metalloproteinase 9single nucleotide polymorphism

More Related Videos

A Magnetic Microbead Occlusion Model to Induce Ocular Hypertension-Dependent Glaucoma in Mice09:26

A Magnetic Microbead Occlusion Model to Induce Ocular Hypertension-Dependent Glaucoma in Mice

Published on: March 23, 2016

20.4K
Quantification of Immunostained Caspase-9 in Retinal Tissue05:18

Quantification of Immunostained Caspase-9 in Retinal Tissue

Published on: July 25, 2022

1.7K

Related Concept Videos

Single Nucleotide Polymorphisms-SNPs01:05

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
17.8K
Glaucoma: Overview01:25

Glaucoma: Overview

Glaucoma is an eye condition characterized by increased intraocular pressure that damages the retina and optic nerve, leading to irreversible blindness if left untreated. The human eye has various components, including the cornea, iris, pupil, lens, and optic nerve. Aqueous humor is secreted by the epithelium of the ciliary body in the posterior chamber and flows through the trabecular meshwork and canal of Schlemm, maintaining normal intraocular pressure. The trabecular meshwork and the canal...
1.2K
Open Angle Glaucoma: Treatment01:27

Open Angle Glaucoma: Treatment

In open-angle glaucoma, the iridocorneal angle remains open, but the trabecular meshwork becomes stiff, slowing down the outflow of aqueous humor. This causes a buildup of aqueous humor in the anterior chamber, leading to a sudden increase in intraocular pressure. The treatment for open-angle glaucoma focuses on reducing the elevated intraocular pressure by either decreasing the secretion of aqueous humor or increasing its outflow.
Drugs such as carbonic anhydrase inhibitors, α2- and...
909
Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...
3.2K