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Updated: Jan 4, 2026
An Aptamer-based Sensor for Unchelated GadoliniumIII
Published on: January 9, 2017
Fluorescent aptasensor based on D-AMA/F-CSC for the sensitive and specific recognition of myoglobin
Dongwei Miao1, Dongkui Liu1, Yanbo Zeng2
1School of Petrochemical Engineering, Changzhou University, Changzhou, 213016, Jiangsu, China; College of Biological, Chemical Sciences and Engineering, Jiaxing University, 118 Jiahang Road, Jiaxing, 314001, Zhejiang, China.
Abstract:
A novel fluorescent biosensor based on dabcyl [(E)-4-((4-(dimethylamino) phenyl) diazenyl)benzoic acid] -modified anti-Mb aptamer (D-AMA) and 6-FAM(6-carboxyfluorescein) -modified complementary short chain (F-CSC)for the specific and sensitive detection of Mb levels is presented in this study. In PBS buffer solution, D-AMA bound to F-CSC, and then dabcyl quenched the fluorescence of 6-FAM. After adding Mb into the system, D-AMA bound to Mb and separated from F-CSC. The fluorescence of 6-FAM was restored after it separated from dabcyl. The assay exhibited high specificity and sensitivity toward Mb, with a low limit of detection of 0.07 ng/mL (S/N = 3) and linear relationships of 0.1-5 ng/mL. It was further applied to detect Mb levels in spiked human blood sera samples.
