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Published on: December 11, 2020
Renal lesions in leptin receptor-deficient medaka (Oryzias latipes)
Shinichi Chisada1, Akihiko Sugiyama2
1Department of Hygiene and Public Health, Kyorin University School of Medicine, 6-20-2 Shinkawa, Mitaka-shi, Tokyo 181-8611, Japan.
Abstract:
The aim of this study was to elucidate the renal lesions of leptin receptor-deficient medaka showing hyperglycemia and hypoinsulinemia and to evaluate the usefulness of the medaka as a model of diabetic nephropathy. Leptin receptor-deficient medaka at 20 and 30 weeks of age showed hyperglycemia and hypoinsulinemia; they also showed a higher level of plasma creatinine than the control medaka. Histopathologically, dilation of glomerular capillary lumina and of afferent/efferent arterioles was observed in leptin receptor-deficient medaka at 20 weeks of age, and then glomerular enlargement with cell proliferation and matrix expansion, formation of fibrin cap-like lesions, glomerular atrophy with Bowman's capsule dilation, and renal tubule dilation were observed at 30 weeks of age. These histopathological characteristics of leptin receptor-deficient medaka were similar to the characteristics of kidney lesions of human and rodent models of type II diabetes mellitus, making leptin receptor-deficient medaka a useful model of diabetic nephropathy.
Insights
Leptin receptor-deficient medaka exhibit hyperglycemia and kidney lesions similar to diabetic nephropathy. This fish model offers a valuable tool for studying diabetes complications and potential treatments.
Area of Science:
- Comparative Pathology
- Endocrinology
- Nephrology
Background:
- Leptin receptor deficiency causes hyperglycemia and hypoinsulinemia.
- Diabetic nephropathy is a major complication of diabetes mellitus.
- Medaka fish offer a potential model for studying kidney diseases.
Purpose of the Study:
- To investigate the renal lesions in leptin receptor-deficient medaka.
- To assess the utility of this medaka model for diabetic nephropathy research.
Main Methods:
- Histopathological examination of medaka kidneys at 20 and 30 weeks.
- Analysis of plasma creatinine levels.
- Comparison with human and rodent diabetic nephropathy models.
Main Results:
- Leptin receptor-deficient medaka displayed hyperglycemia, hypoinsulinemia, and elevated plasma creatinine.
- Kidney lesions included glomerular capillary and arteriole dilation, glomerular enlargement, cell proliferation, matrix expansion, fibrin cap-like lesions, glomerular atrophy, and tubule dilation.
- These findings resemble kidney pathologies observed in human and rodent type II diabetes.
Conclusions:
- Leptin receptor-deficient medaka develop renal lesions analogous to diabetic nephropathy.
- This medaka model is a promising tool for investigating the pathogenesis and treatment of diabetic kidney disease.
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