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Rapid Isolation of Single Cells from Mouse and Human Teeth
Published on: October 28, 2021
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Tooth bioengineering from single cell suspensions.
Vassilis Stratoulias1, Frederic Michon1,2
1Institute of Biotechnology, Helsinki Institute of Life Science, Developmental Biology Program, University of Helsinki, 00790, Helsinki, Finland.
Methodsx
|November 14, 2019
Summary
Researchers developed a method to bioengineer a tooth from single cell suspensions. This technique also allows for processing the tooth for histological analysis, enabling further research applications.
Area of Science:
- Bioengineering
- Developmental Biology
- Biomaterials Science
Background:
- Recent advances in bioengineering, biomaterials, developmental biology, and stem cell research enable organ replacement.
- Functional organ replacement is becoming increasingly feasible.
Purpose of the Study:
- To describe a methodology for bioengineering a tooth from single cell suspensions.
- To outline subsequent processing steps for minute structures, such as teeth, for various analytical applications.
Main Methods:
- Bioengineering a tooth using embryonic epithelial and mesenchymal single cell suspensions.
- Processing the bioengineered tooth for histological examination, immunofluorescence, and in situ hybridization.
- Ensuring single cell populations for reliable results.
Main Results:
- A detailed methodology for reproducible and reliable bioengineering of a tooth.
- Successful processing of the minute bioengineered structure for downstream analyses.
- The described methodology is applicable to other minute structures requiring paraffin embedding.
Conclusions:
- The presented methodology offers a reliable approach for bioengineering teeth.
- This technique facilitates further research by enabling detailed histological and molecular analysis of bioengineered teeth.
- The method's adaptability extends its utility to various minute biological structures.
