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CircZNF609/miR-134-5p/BTG-2 axis regulates proliferation and migration of glioma cell
Hui Tong1, Kai Zhao2, Jiangjie Wang1
1Department of Neurosurgery, Linyi Central Hospital, Linyi, China.
Objectives:
MicroRNAs are abundant in eukaryotic cells and play key roles in cancers. Circular RNAs (CircRNAs) served as the competing endogenous RNAs (ceRNAs) in mediating multiple cell processes. This study aims to define the role of CircRNA CircZNF609/miR-134-5p in glioma as well as the underlying regulating mechanism.
Methods:
Relative expression of miR-134-5p, CircZNF609 and BTG-2 mRNA was determined by quantitative real-time PCR. Cell proliferation was analysed by CCK-8 assay. Cell migration was assessed by cell wound scratch assay. The direct regulatory of miR-134-5p on BTG-2 and CircZNF609 was verified by luciferase report gene assay.
Key Findings:
MiR-134-5p was significantly upregulated in glioma cells. The overexpression of miR-134-5p inhibited cell proliferation and migration of glioma cell U251 and U87. Reversely, knock-down of miR-134-5p enhanced cell proliferation and migration. Both BTG-2 and CircZNF609 are the direct targets of miR-134-5p, and their expression could be negatively regulated by miR-134-5p. CircZNF609 was significantly upregulated in U251 and U87 cells and acted as an oncogene to promote cell proliferation and cell migration of glioma cell U251 and U87.
Conclusions:
These data proved that CircZNF609 served as a competing RNA to bind miR-134-5p that promoted BTG-2 expression leading to reduced proliferation and migration of glioma cell.
Insights
Circular RNA CircZNF609 acts as an oncogene in glioma by sponging miR-134-5p, promoting cell proliferation and migration. This interaction ultimately leads to reduced BTG-2 expression, impacting glioma progression.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- MicroRNAs and circular RNAs (CircRNAs) are crucial in cancer development.
- CircRNAs function as competing endogenous RNAs (ceRNAs) regulating cellular processes.
- Understanding CircRNA roles in glioma is vital for therapeutic strategies.
Purpose of the Study:
- To investigate the role of CircRNA CircZNF609/miR-134-5p in glioma.
- To elucidate the underlying regulatory mechanism of CircZNF609 and miR-134-5p in glioma cells.
Main Methods:
- Quantitative real-time PCR for gene expression analysis.
- Cell proliferation assays (CCK-8) and migration assays (wound scratch).
- Luciferase reporter assays to confirm direct target interactions.
Main Results:
- MiR-134-5p was upregulated in glioma cells, inhibiting proliferation and migration.
- CircZNF609 and BTG-2 were identified as direct targets of miR-134-5p.
- CircZNF609 was upregulated in glioma cells, promoting proliferation and migration.
Conclusions:
- CircZNF609 acts as a ceRNA, binding miR-134-5p.
- This interaction promotes BTG-2 expression, reducing glioma cell proliferation and migration.
- CircZNF609 functions as an oncogene in glioma progression.
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