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Updated: Jan 3, 2026

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

In Vitro Differentiation of Human CD4⁺FOXP3⁺ Induced Regulatory T Cells (iTregs) from Naïve CD4⁺ T Cells Using a TGF-β-containing Protocol

Published on: December 30, 2016

IL-10-Producing Potential Treg Precursor in Placenta.

¹Department of Immunology, Kurume University of School of Medicine.

The Kurume Medical Journal

|November 15, 2019

Summary

Researchers discovered novel IL-10-producing cells in the placenta, crucial for maternal-fetal immune tolerance. These unique CD19-, CD3-, B220+ cells may be precursors to regulatory T cells (Treg), supporting reproductive success.

Keywords:

Breg, Treg hematopoietic stem cell immune tolerance placenta

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Last Updated: Jan 3, 2026

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

In Vitro Differentiation of Human CD4⁺FOXP3⁺ Induced Regulatory T Cells (iTregs) from Naïve CD4⁺ T Cells Using a TGF-β-containing Protocol

Published on: December 30, 2016

Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface

Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface

Published on: May 21, 2015

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

Area of Science:

Immunology
Reproductive Biology
Cell Biology

Background:

Maternal immune tolerance to allogeneic fetal tissue is essential for successful reproduction.
The regulatory immune cell network is critical for maintaining this maternal-fetal tolerance.

Purpose of the Study:

To identify and characterize novel immune cells involved in maternal-fetal immune tolerance.
To investigate the role of Interleukin-10 (IL-10) producing cells in the placenta.

Main Methods:

Utilized a green fluorescent protein (GFP)/IL-10 reporter mouse model.
Employed flow cytometry to analyze cell surface markers (CD19, CD3, B220).
Conducted in vitro culture and adoptive cell transfer experiments.

Main Results:

Identified unique IL-10-expressing cells in the placenta with a CD19-, CD3-, B220+ phenotype.
These cells demonstrated self-renewing properties and were enriched in vitro.
Detected IL-10 production in Sca-1+, CD4+, CD25+ regulatory T cells (Treg) following adoptive transfer.

Conclusions:

This study reports the first identification of IL-10-producing CD19-, CD3-, B220+ cells in the fetus/placenta.
These cells are potential progenitors for Sca-1+ regulatory T cells (Treg).
These findings offer insights into immune tolerance mechanisms during pregnancy and potential pluripotent immune precursor cells.