Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jan 3, 2026

Development of Combinatorial Therapeutics for Spinal Cord Injury using Stem Cell Delivery

Development of Combinatorial Therapeutics for Spinal Cord Injury using Stem Cell Delivery

Published on: June 7, 2024

Novel Sodium Deoxycholate-Based Chemical Decellularization Method for Peripheral Nerve.

Michaela W McCrary¹, Natalie E Vaughn¹, Nora Hlavac¹

¹J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida.

Tissue Engineering. Part C, Methods

|November 15, 2019

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Correction: Injectable hydrogel-based localized delivery of IDO-galectin-3 mitigates neuroinflammation and promotes neuronal sparing after spinal cord contusion in rats.

Journal of materials chemistry. B·2026

Same author

Tuning hydrogel properties and Schwann cell behavior through microchannel size control in magnetically templated hydrogels.

Biomaterials science·2026

Same author

Injectable hydrogel-based localized delivery of IDO-galectin-3 mitigates neuroinflammation and promotes neuronal sparing after spinal cord contusion in rats.

Journal of materials chemistry. B·2026

Same author

Comparative Analysis of Matrigel and Tunable Collagen-Fibrin Blends for in Vitro Skeletal Muscle Models.

Journal of biomedical materials research. Part A·2026

Same author

Patterning of anisotropic physical cues in granular PEG hydrogel composites using magnetic templating.

Industrial & engineering chemistry research·2026

Same author

Unique electrochemically synthesized polypyrrole:poly(lactic-<i>co</i>-glycolic acid) blends for biomedical applications.

Journal of materials chemistry·2026

A new decellularization method using sodium deoxycholate (SDC) effectively removes cells from peripheral nerves while preserving extracellular matrix (ECM). This novel method offers a viable alternative for nerve repair and spinal cord injury applications.

Area of Science:

Biomaterials Science
Regenerative Medicine
Tissue Engineering

Background:

Decellularized peripheral nerve scaffolds are crucial for nerve repair and spinal cord injury treatment.
The established Hudson method relies on discontinued Triton X-200, necessitating a new decellularization technique.
Existing methods lack efficient cell removal while preserving native extracellular matrix (ECM) structure.

Purpose of the Study:

To optimize a novel chemical decellularization method for peripheral nerves using readily available sodium deoxycholate (SDC).
To evaluate the efficacy of the new method in removing cellular components and preserving ECM.
To assess the biocompatibility and proteomic profile of the resulting decellularized nerve scaffolds.

Main Methods:

Rat sciatic nerves underwent sequential washes with buffers, zwitterionic detergents, and varying concentrations of SDC.

Keywords:

acellular matrices decellularization peripheral nerve sodium deoxycholate

More Related Videos

A Decellularization Methodology for the Production of a Natural Acellular Intestinal Matrix

A Decellularization Methodology for the Production of a Natural Acellular Intestinal Matrix

Published on: October 7, 2013

Purification of Fibroblasts and Schwann Cells from Sensory and Motor Nerves in Vitro

Purification of Fibroblasts and Schwann Cells from Sensory and Motor Nerves in Vitro

Published on: May 20, 2020

Related Experiment Videos

Last Updated: Jan 3, 2026

Development of Combinatorial Therapeutics for Spinal Cord Injury using Stem Cell Delivery

Development of Combinatorial Therapeutics for Spinal Cord Injury using Stem Cell Delivery

Published on: June 7, 2024

A Decellularization Methodology for the Production of a Natural Acellular Intestinal Matrix

A Decellularization Methodology for the Production of a Natural Acellular Intestinal Matrix

Published on: October 7, 2013

Purification of Fibroblasts and Schwann Cells from Sensory and Motor Nerves in Vitro

Purification of Fibroblasts and Schwann Cells from Sensory and Motor Nerves in Vitro

Published on: May 20, 2020

Deoxyribonuclease (DNase) incubation and salt buffer washes were employed to optimize DNA removal.

Immunohistochemistry, proteomic analysis, and cytotoxicity assays were performed to characterize the scaffolds.

Main Results:

3% SDC effectively removed Schwann cells, axons, and myelin while preserving ECM components and structure.
A 3-hour DNase incubation significantly reduced cellular debris compared to the Hudson method.
The novel SDD method demonstrated a similar proteomic profile to the Hudson method with fewer cellular proteins and showed no significant cytotoxicity.
Decellularized scaffolds supported metabolically active Schwann cells in vitro.

Conclusions:

The novel SDD decellularization method using SDC, DNase, zwitterionic detergents, and salt buffers is a viable alternative to the discontinued Hudson method.
This method effectively removes cellular components while preserving the native ECM, yielding biocompatible scaffolds for regenerative applications.
The optimized SDD method provides a clinically relevant approach for producing decellularized nerve grafts for peripheral nerve repair and spinal cord injury treatment.