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Updated: Jan 3, 2026
A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants
Published on: December 16, 2015
Chromosome Preparation in Rice (Oryza sativa)
1Center for Genomics and Biotechnology, Fujian Agriculture and Forestry University, Fujian, China.
Abstract:
Chromosomes are the carriers of genetic material in biological organisms. Each chromosome has three essential components: a centromere, telomeres, and chromosome arms. Chromosome preparation is the basic technique for studies in cytogenetics, including the analysis of chromosomal behavior, chromosomal variation, karyotyping analysis, fluorescence in situ hybridization, immunostaining, and artificial chromosome technology. In this unit, we describe the basic protocol for rice (Oryza sativa) metaphase chromosome preparation from rice root tips, alternative chromosome preparation protocols using cultured cells, and different chromosome spreading techniques. © 2016 by John Wiley & Sons, Inc.
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