Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Homologous Recombination02:31

Homologous Recombination

62.2K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
62.2K
Homologous Recombination02:31

Homologous Recombination

5.9K
5.9K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

16.4K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
16.4K
DNA Base Pairing02:27

DNA Base Pairing

32.7K
Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
32.7K
DNA Base Pairing02:27

DNA Base Pairing

31.3K
31.3K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

14.2K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
14.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The fungal RIP hypermutator mechanism has deep eukaryotic roots.

Trends in genetics : TIG·2026
Same author

Remodeling of perturbed chromatin can initiate de novo transcriptional and post-transcriptional silencing.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same author

C-DNA may facilitate homologous DNA pairing.

Trends in genetics : TIG·2023
Same author

A gene cluster with positive and negative elements controls bistability and hysteresis of the crippled versus normal growth in the fungus Podospora anserina.

Fungal genetics and biology : FG & B·2022
Same author

Modulation of C-to-T mutation by recombination-independent pairing of closely positioned DNA repeats.

Biophysical journal·2021
Same author

Recombination-independent recognition of DNA homology for meiotic silencing in <i>Neurospora crassa</i>.

Proceedings of the National Academy of Sciences of the United States of America·2021

Related Experiment Video

Updated: Jan 3, 2026

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

2.1K

Direct Homologous dsDNA-dsDNA Pairing: How, Where, and Why?

Alexey K Mazur1, Tinh-Suong Nguyen2, Eugene Gladyshev2

  • 1CNRS, Université de Paris, UPR 9080, Laboratoire de Biochimie Théorique, 13 Rue Pierre et Marie Curie, F-75005 Paris, France; Group Fungal Epigenomics, Department of Mycology, Institut Pasteur, Paris 75015, France; Institut de Biologie Physico-Chimique-Fondation Edmond de Rothschild, PSL Research University, Paris, France.

Journal of Molecular Biology
|November 15, 2019
PubMed
Summary

Homologous chromosomes pair without DNA breakage, a process crucial for eukaryotic biology. Fungal studies reveal direct DNA pairing mechanisms independent of standard recombination proteins.

Keywords:
DNA homology recognitionMSUDRIPRecombination-independentdsDNA–dsDNA pairing

More Related Videos

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
06:24

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Published on: February 13, 2019

8.4K
Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
06:59

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

2.8K

Related Experiment Videos

Last Updated: Jan 3, 2026

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

2.1K
Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
06:24

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Published on: February 13, 2019

8.4K
Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
06:59

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

2.8K

Area of Science:

  • Molecular Biology
  • Genetics
  • Eukaryotic Cell Biology

Background:

  • Homologous chromosome pairing is a fundamental eukaryotic process.
  • The mechanism for recombination-independent pairing and homology recognition remains unclear.
  • Previous research suggests sequence homology can be detected between intact DNA double helices in vivo.

Purpose of the Study:

  • To investigate the mechanism of homology recognition in recombination-independent DNA pairing.
  • To explore the role of direct homologous double-stranded DNA (dsDNA)-dsDNA pairing in biological processes.

Main Methods:

  • Analysis of two fungal silencing phenomena: repeat-induced point mutation (RIP) and meiotic silencing by unpaired DNA (MSUD).
  • Genetic evidence was gathered to support direct homologous dsDNA-dsDNA pairing.

Main Results:

  • RIP and MSUD provide genetic evidence for direct homologous dsDNA-dsDNA pairing.
  • This pairing mechanism likely uses a search strategy matching dsDNA segments via base-pair triplets.
  • The process is efficient and occurs independently of RecA/Rad51/Dmc1 proteins.

Conclusions:

  • Direct homologous dsDNA-dsDNA pairing is a viable mechanism for homology recognition.
  • This process, observed in fungal silencing, operates without canonical recombination proteins.
  • Further research into RIP and MSUD could reveal novel DNA functions.