Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

16.4K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
16.4K
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

12.0K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
12.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The mTOR signaling pathway regulates key steps of mammary gland organoid genesis in a temporal manner.

Scientific reports·2026
Same author

Proteasome Cap Targeting Chimeras for Ubiquitination-Independent Targeted Protein Degradation.

Angewandte Chemie (International ed. in English)·2026
Same author

Protocol for dissecting the aggregation-prone protein interactome with optogenetic-induced aggregation and biotin labeling proximity assay.

STAR protocols·2025
Same author

Water Networks as Hydrophobic Recognition Motifs in Proteins.

Angewandte Chemie (International ed. in English)·2025
Same author

Bromodomain proteins IBD1 and IBD2 link histone acetylation to SWR1- and INO80-mediated H2A.Z regulation in Tetrahymena.

Epigenetics & chromatin·2025
Same author

Histone H3 N-terminal recognition by the PHD finger of PHRF1 is required for proper DNA damage response.

Nucleic acids research·2025

Related Experiment Video

Updated: Jan 3, 2026

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
07:41

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

Published on: October 2, 2017

8.8K

Emerging tools to investigate bromodomain functions.

Pata-Eting Kougnassoukou Tchara1, Panagis Filippakopoulos2, Jean-Philippe Lambert1

  • 1Department of Molecular Medicine and Cancer Research Centre, Université Laval, Québec, QC, Canada; Research Center CHU de Québec-Université Laval, Québec, QC G1V 4G2, Canada.

Methods (San Diego, Calif.)
|November 15, 2019
PubMed
Summary

Bromodomains (BRDs) are epigenetic readers crucial for gene regulation. New tools are advancing the study of BRD functions and their roles in disease, aiding therapeutic target development.

Keywords:
Acetyl lysineBromodomainChromatinMass spectrometryProtein-protein interactionsProteomics

More Related Videos

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments
09:14

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

Published on: January 27, 2016

20.0K
Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA
11:35

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA

Published on: August 21, 2016

13.4K

Related Experiment Videos

Last Updated: Jan 3, 2026

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
07:41

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

Published on: October 2, 2017

8.8K
DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments
09:14

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

Published on: January 27, 2016

20.0K
Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA
11:35

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA

Published on: August 21, 2016

13.4K

Area of Science:

  • Biochemistry
  • Epigenetics
  • Molecular Biology

Background:

  • Bromodomains (BRDs) recognize acetylated lysine, an epigenetic mark on histones.
  • BRD-containing proteins are involved in chromatin remodeling and gene expression regulation.
  • Dysfunctional BRD proteins are implicated in various diseases, making them therapeutic targets.

Purpose of the Study:

  • To review emerging tools for studying Bromodomain (BRD) biology.
  • To highlight methods for screening BRD targets and identifying interactors.
  • To discuss the role of proteomics in advancing BRD research.

Main Methods:

  • Structural biology techniques for BRD definition.
  • Functional characterization assays.
  • Interactome mapping (affinity purification, proximity-based biotinylation).
  • Proteomic approaches.

Main Results:

  • Advancements in structural and functional characterization of BRDs.
  • Systematic screening methods for BRD targets identified.
  • Interactome mapping elucidated BRD functions and signaling pathways.
  • Proteomics offers enhanced understanding of BRD biology.

Conclusions:

  • Emerging tools have significantly advanced the understanding of Bromodomain (BRD) biology.
  • Interactome mapping and proteomics are key to elucidating BRD functions and disease involvement.
  • Continued research using these tools promises further insights into BRD-mediated pathways and therapeutic strategies.