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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

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Related Experiment Video

Updated: Jan 3, 2026

Protocols for C-Brick DNA Standard Assembly Using Cpf1

Protocols for C-Brick DNA Standard Assembly Using Cpf1

Published on: June 15, 2017

CRATES: A one-step assembly method for Class 2 CRISPR arrays.

Chunyu Liao¹, Rebecca A Slotkowski², Chase L Beisel³

¹Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC, United States; Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), Würzburg, Germany.

Methods in Enzymology

|November 16, 2019

Summary

This summary is machine-generated.

CRISPR Assembly through Trimmed Ends of Spacers (CRATES) simplifies the creation of large CRISPR arrays. This new method enables efficient multiplexed targeting for CRISPR technologies.

Failed At:

2026-06-19T13:38:26.563040+00:00

Keywords:

CRATES CRISPR array DNA assembly Multiplexing

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