Performance of enhanced anaerobic digestion with different pyrolysis biochars and microbial communities

Anaerobic digestion (AD) is commonly used to treat biowastes, however, there are challenges in AD such as low methane yield, intermediate inhibition, and system instability. In this study, the effects of typical biochars on methane yield and microbial variation for AD with straw and cow manure were explored. The results indicated that cumulative methane yield with coconut shell biochar was higher than that without a biochar (319.44 vs. 282.77 mL/g VS). Interestingly, AD with biochars had a secondary methane yield peak, whereas control groups did not show this phenomenon. A suitable dosage (e.g., straw biochar of 2%) improved cumulative methane yield, but excessive addition (4%) could inhibit AD. AD system with biochar was more helpful for the growth of acetoclastic methanogens rather than hydrogenotrophic methanogens. The study demonstrated biochar can indeed enhance AD performance, and microbial community analyses could supply valuable information to elucidate the mechanism of enhancement.