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Related Experiment Video

Updated: Nov 9, 2025

Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells

Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells

Published on: June 5, 2020

Hepatitis E virus replication in human intestinal cells.

Olivier Marion^1,2,3, Sebastien Lhomme^2,3,4, Manon Nayrac²

¹Department of Nephrology and Organs Transplantation, Toulouse Rangueil University Hospital, Toulouse, France.

|November 16, 2019

Summary

This summary is machine-generated.

Hepatitis E virus (HEV) can replicate within human intestinal cells. This study shows HEV is released from these cells as quasi-enveloped virions, explaining how it reaches the liver after ingestion.

Keywords:

antiviral chemotherapy enteric infections epithelial cells hepatitis E

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Last Updated: Nov 9, 2025

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Area of Science:

Virology
Gastroenterology
Hepatology

Background:

Hepatitis E virus (HEV) is a major cause of acute hepatitis globally.
Transmission occurs via contaminated water (HEV genotypes 1 and 2) or undercooked meat (HEV genotypes 3 and 4).
The pathway of HEV from ingestion to the liver, and its replication in intestinal cells, remains poorly understood.

Purpose of the Study:

To investigate the replication of HEV in human intestinal cells.
To determine the form in which HEV is released from intestinal cells.
To explore the potential role of intestinal cells in HEV infection.

Main Methods:

Development of human primary small intestine epithelial cell cultures and intestinal explants.
Polarization of epithelial cells on transwells for directional release studies.
Infection of cells and explants with HEV strains and characterization of released virions.

Main Results:

Primary intestinal cells supported HEV replication.
Polarized enterocytes released HEV particles primarily apically, with some basal release.
HEV virions released from enterocytes were quasi-enveloped (density 1.06-1.08 g/cm³).
Ribavirin inhibited basal HEV excretion but not apical release.

Conclusions:

HEV can replicate in human intestinal epithelial cells.
Quasi-enveloped HEV particles are released from intestinal cells, facilitating liver infection.
Intestinal cells play a significant role in the HEV life cycle.