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Complete Genome Sequences of Seven EA Cluster Microbacteriophages, Bustleton, MillyPhilly, Riyhil, Phriends, Pherbot,

Anh M Nguyen Quach1, Amy E Varghese1, Kumal Siddiq1

  • 1Department of Biology, Drexel University, Philadelphia, Pennsylvania, USA.

Microbiology Resource Announcements
|November 16, 2019
PubMed

Insights

Researchers isolated seven EA cluster microbacteriophages from Philadelphia soil using Microbacterium foliorum. These phages share a conserved genome, with variations in the 3' end, including an uncharacterized gene in the Phriends phage.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Microbacteriophages are viruses that infect bacteria.
  • The EA cluster is a group of related microbacteriophages.
  • Understanding phage diversity is crucial for various applications.

Purpose of the Study:

  • To isolate and characterize novel EA cluster microbacteriophages.
  • To investigate the genomic structure and diversity of these phages.
  • To identify unique genetic elements within the phage genomes.

Main Methods:

  • Isolation of phages from soil samples.
  • Bacterial host: Microbacterium foliorum.
  • Genome sequencing and comparative analysis.

Main Results:

  • Seven EA cluster microbacteriophages were successfully isolated.
  • All isolated phages exhibited a highly conserved genome.
  • Regions of genomic diversity were localized to the 3' end.
  • The Phriends phage (EA1 cluster) possesses an orpham gene of unknown function at the 3' end.

Conclusions:

  • The EA cluster phages share a conserved genomic backbone with localized diversity.
  • The orpham gene in Phriends represents a novel genetic element requiring further investigation.
  • These findings contribute to the understanding of phage genomics and evolution.

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