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Updated: Jan 3, 2026

Experimental Protocol for Manipulating Plant-induced Soil Heterogeneity
Published on: March 13, 2014
The role of multiple global change factors in driving soil functions and microbial biodiversity
Matthias C Rillig1,2, Masahiro Ryo3,2, Anika Lehmann3,2
1Institute of Biology, Freie Universität Berlin, 14195 Berlin, Germany. rillig@zedat.fu-berlin.de.
Abstract:
Soils underpin terrestrial ecosystem functions, but they face numerous anthropogenic pressures. Despite their crucial ecological role, we know little about how soils react to more than two environmental factors at a time. Here, we show experimentally that increasing the number of simultaneous global change factors (up to 10) caused increasing directional changes in soil properties, soil processes, and microbial communities, though there was greater uncertainty in predicting the magnitude of change. Our study provides a blueprint for addressing multifactor change with an efficient, broadly applicable experimental design for studying the impacts of global environmental change.
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