Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.8K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.8K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Size estimation of mixed free-floating sub-resolution spherical scatterers utilizing adaptive scanning optical coherence tomography.

Optics continuum·2026
Same author

Corneal Innervation Research at a Crossroads: A Tool-Driven Roadmap for the Future.

Investigative ophthalmology & visual science·2026
Same author

Distinguishing Factors for Microbial Keratitis Groups: A Cross-Sectional Survey of US Cornea Specialists.

Cornea·2026
Same author

Robust registration under large image misalignment using an iterative step-aware transformer with application to corneal confocal microscopy.

Biomedical optics express·2026
Same author

Spatial Coherence Loss: All Objects Matter in Salient and Camouflaged Object Detection.

Pattern recognition·2026
Same author

Early Treatment Response and 90-Day Vision in Microbial Keratitis.

JAMA ophthalmology·2026

Related Experiment Video

Updated: Jan 3, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

8.9K

Super-resolution retinal imaging using optically reassigned scanning laser ophthalmoscopy.

Theodore B DuBose1, Francesco LaRocca1, Sina Farsiu1,2

  • 1Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA.

Nature Photonics
|November 16, 2019
PubMed
Summary

This study introduces advanced optical reassignment for faster, reflective super-resolution imaging. The new method enables high-resolution in vivo imaging of human retinal cone cells without specialized equipment.

More Related Videos

Subretinal Transplantation of Human Embryonic Stem Cell Derived-retinal Pigment Epithelial Cells into a Large-eyed Model of Geographic Atrophy
11:03

Subretinal Transplantation of Human Embryonic Stem Cell Derived-retinal Pigment Epithelial Cells into a Large-eyed Model of Geographic Atrophy

Published on: January 22, 2018

10.4K
Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research
10:10

Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research

Published on: November 2, 2018

9.7K

Related Experiment Videos

Last Updated: Jan 3, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

8.9K
Subretinal Transplantation of Human Embryonic Stem Cell Derived-retinal Pigment Epithelial Cells into a Large-eyed Model of Geographic Atrophy
11:03

Subretinal Transplantation of Human Embryonic Stem Cell Derived-retinal Pigment Epithelial Cells into a Large-eyed Model of Geographic Atrophy

Published on: January 22, 2018

10.4K
Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research
10:10

Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research

Published on: November 2, 2018

9.7K

Area of Science:

  • Physics
  • Optics
  • Biomedical Imaging

Background:

  • Super-resolution microscopy has revolutionized scientific discovery but often relies on fluorescence, limiting applications in reflective imaging.
  • Existing optical reassignment techniques are slow and primarily suited for fluorescence imaging.

Purpose of the Study:

  • To adapt optical reassignment for broader scanning microscopy applications, including reflective imaging.
  • To significantly increase the speed of optical reassignment imaging.
  • To develop a super-resolution in vivo imaging device for the human retina.

Main Methods:

  • Developed novel advances in optical reassignment technology.
  • Adapted the technique for any scanning microscopy modality.
  • Implemented optically reassigned scanning laser ophthalmoscopy for in vivo retinal imaging.

Main Results:

  • Achieved an order of magnitude faster image acquisition compared to previous optical reassignment methods.
  • Enabled super-resolution reflective imaging.
  • Successfully imaged living human retinal cone photoreceptor cells in vivo.
  • Demonstrated high-resolution retinal imaging without adaptive optics or pupil dilation.

Conclusions:

  • The enhanced optical reassignment technique broadens the applicability of super-resolution microscopy to reflective imaging modalities.
  • Optically reassigned scanning laser ophthalmoscopy provides a non-invasive, high-resolution method for in vivo human retinal imaging.
  • This advancement offers new possibilities for studying retinal diseases and vision science.