Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Simple microcomputer system for mapping tissue sections with the light microscope.

A Alvarez-Buylla1, D S Vicario

  • 1Rockefeller University, New York, NY 10021.

Journal of Neuroscience Methods
|September 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Usefulness of MALDI-TOF and REP-PCR against PFGE for the epidemiological study of Acinetobacter baumannii].

Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia·2021
Same author

Influence of early-life nutritional stress on songbird memory formation.

Proceedings. Biological sciences·2018
Same author

Enhanced surveillance of carbapenemase-producing Gram-negative bacteria to support national and international prevention and control efforts.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2016
Same author

Exposure to a novel stimulus environment alters patterns of lateralization in avian auditory cortex.

Neuroscience·2014
Same author

The heterogeneity of adult neural stem cells and the emerging complexity of their niche.

Cold Spring Harbor symposia on quantitative biology·2008
Same author

Primary cilia are required for cerebellar development and Shh-dependent expansion of progenitor pool.

Developmental biology·2008
Same journal

Time as the language of Behavior: events, sequences, patterns and meanings.

Journal of neuroscience methods·2026
Same journal

Detection of cochlear microphonic for differential diagnosis between auditory neuropathy mice and noise-induced sensorineural hearing loss mice.

Journal of neuroscience methods·2026
Same journal

Assessment metrics for pain control in rats: A methodological commentary.

Journal of neuroscience methods·2026
Same journal

Infant EEG preprocessing pipelines: A capability framework and current gaps in practice.

Journal of neuroscience methods·2026
Same journal

Methods for measuring neural activity during voluntary wheel running.

Journal of neuroscience methods·2026
Same journal

Serotype-dependent differences in AAV cellular transduction rates in the hypothalamus of Arctic ground squirrels.

Journal of neuroscience methods·2026
See all related articles

This study introduces a novel 2D tissue mapping method using a drawing tube and microcomputer. It significantly reduces time and effort for detailed anatomical feature mapping, enabling digital analysis.

Area of Science:

  • Neuroscience
  • Histology
  • Biotechnology

Background:

  • Accurate two-dimensional mapping of tissue sections is crucial for anatomical and cellular analysis.
  • Traditional methods are often time-consuming and labor-intensive, limiting high-resolution data acquisition and statistical analysis.

Purpose of the Study:

  • To develop and present an efficient method for high-resolution two-dimensional mapping of tissue sections.
  • To enable rapid data acquisition, editing, and statistical analysis of anatomical features.

Main Methods:

  • A novel system integrating a drawing tube, microscope stage encoders, and a microcomputer for real-time specimen mapping.
  • Direct visualization of the specimen with superimposed digital cursor and menus for precise landmark identification.
  • Utilizing a mouse for data entry and program control, with automated storage of coordinate values.

Related Experiment Videos

Main Results:

  • The system allows for rapid mapping of various anatomical features, including cell positions and stained regions, directly through the microscope without stage movement.
  • Generated maps can include line drawings and up to 20 landmark types with stored, editable coordinate values.
  • Specialized functions like grain counting and area calculations are integrated, producing high-resolution plots.

Conclusions:

  • This method significantly reduces the time and effort required for detailed tissue mapping compared to conventional techniques.
  • The digital format of the mapped data facilitates subsequent statistical analysis and maintains high resolution.
  • The system employs widely available hardware, making it a practical tool for diverse research applications in histology and neuroscience.