Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

8.2K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
8.2K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.0K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Association between genetic polymorphism of tumor necrosis factor and chronic severe hepatitis B in patients].

Zhonghua yi xue za zhi·2007
Same author

In vivo translational inaccuracy in Escherichia coli: missense reporting using extremely low activity mutants of Vibrio harveyi luciferase.

Biochemistry·2007
Same author

[Construction of recombinant adenovirus vector expressing extracellular domain of TbetaR-II-RANTES fusion gene and its anti-tumor effects].

Zhonghua zhong liu za zhi [Chinese journal of oncology]·2007
Same author

[Characteristics, evolution and variation of M genes of human avian H5N1 strains in Guangdong].

Bing du xue bao = Chinese journal of virology·2007
Same author

Dynamic changes in microbial activity and community structure during biodegradation of petroleum compounds: a laboratory experiment.

Journal of environmental sciences (China)·2007
Same author

Differences in optical transport properties between human meridian and non-meridian.

The American journal of Chinese medicine·2007

Related Experiment Video

Updated: Jan 3, 2026

Identification of Functional Protein Regions Through Chimeric Protein Construction
11:39

Identification of Functional Protein Regions Through Chimeric Protein Construction

Published on: January 8, 2019

10.9K

Validation of Chimeric Fusion Peptides Using Proteomics Data.

Sandeep Singh1, Hui Li2

  • 1Department of Pathology, School of Medicine, University of Virginia, Charlottesville, VA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 16, 2019
PubMed
Summary
This summary is machine-generated.

Identifying fused proteins from chimeric RNAs is crucial for therapeutic applications. This study details methods for validating these chimeric proteins using public proteomics data and in silico digestion techniques.

Keywords:
Chimeric/fusion protein identificationIn silico peptide digestionProteomics

More Related Videos

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
11:54

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Published on: March 23, 2020

10.2K
Identifying Protein-protein Interaction Sites Using Peptide Arrays
07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

18.5K

Related Experiment Videos

Last Updated: Jan 3, 2026

Identification of Functional Protein Regions Through Chimeric Protein Construction
11:39

Identification of Functional Protein Regions Through Chimeric Protein Construction

Published on: January 8, 2019

10.9K
Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
11:54

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Published on: March 23, 2020

10.2K
Identifying Protein-protein Interaction Sites Using Peptide Arrays
07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

18.5K

Area of Science:

  • Biochemistry
  • Bioinformatics
  • Proteomics

Background:

  • Chimeric RNAs and their fused protein products hold significant therapeutic potential, impacting diagnostics and drug targeting.
  • While algorithms for identifying chimeric RNAs are established, validating their corresponding fused proteins remains an emerging challenge.

Purpose of the Study:

  • To outline a comprehensive methodology for the identification and validation of fused proteins derived from chimeric RNAs.
  • To provide a framework for leveraging publicly available proteomics datasets for chimeric protein discovery.

Main Methods:

  • Detailed protocols for downloading and pre-processing public proteomics datasets.
  • Development of a fusion peptide database via in silico tryptic digestion of known chimeric proteins.
  • Utilization of specific software tools for the identification of chimeric peptides within complex proteomics data.

Main Results:

  • Established a systematic workflow for validating chimeric proteins.
  • Demonstrated the feasibility of identifying chimeric peptides in large-scale proteomics datasets.
  • Provided a reproducible method for researchers in the field.

Conclusions:

  • The validation of chimeric proteins through proteomics data analysis is an accessible and vital step in harnessing their therapeutic potential.
  • This methodology facilitates the discovery and characterization of novel chimeric proteins for further research and development.
  • The described approach aids in bridging the gap between chimeric RNA identification and functional protein validation.