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C-MET-dependent signal transduction mediates retinoblastoma growth by regulating PKM2 nuclear translocation

Hanjun Dai1, Weijuan Zeng1, Hong Luo1

  • 1Department of Ophthalmology, Zhongnan Hospital of Wuhan University, Wuhan, People's Republic of China.

Cell Biochemistry and Function
|November 16, 2019
PubMed

Insights

C-MET factor is highly expressed in retinoblastoma cells, driving proliferation. Inhibiting C-MET (mesenchymal-epithelial transition factor) suppressed tumor growth, revealing a new therapeutic target for retinoblastoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Mesenchymal-epithelial transition (C-MET) factor is overexpressed in many cancers, making it a key therapeutic target.
  • The role of C-MET in retinoblastoma, a significant eye cancer, is not well understood.

Purpose of the Study:

  • To investigate the role and mechanism of C-MET in Y79 retinoblastoma cells.
  • To determine if C-MET is a potential therapeutic target for retinoblastoma.

Main Methods:

  • Investigated C-MET expression in Y79 retinoblastoma cells.
  • Assessed the impact of C-MET inhibition on cell proliferation and tumor growth.
  • Elucidated the downstream signaling pathway involving ERK1/2, PKM2, and histone H3.

Main Results:

  • C-MET was highly expressed in Y79 retinoblastoma cells, correlating positively with proliferation and tumor growth.
  • Inhibition of C-MET significantly suppressed retinoblastoma cell proliferation and tumor growth.
  • Identified a signaling cascade: HGF-induced C-MET activates ERK1/2, promoting PKM2 nuclear translocation, which interacts with histone H3 to drive cyclin D1 and c-Myc expression.

Conclusions:

  • C-MET plays a crucial role in promoting retinoblastoma cell proliferation and tumor growth.
  • A novel C-MET-dependent signaling pathway (ERK1/2/PKM2/histone H3) contributes to retinoblastoma progression.
  • C-MET represents a promising therapeutic target for retinoblastoma treatment.
Keywords:
C-METERK 1/2PKM2retinoblastomatumour growth

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