Related Experiment Video
Updated: Jan 3, 2026

Scanning Electron Microscopy SEM Protocols for Problematic Plant, Oomycete, and Fungal Samples
Published on: February 3, 2017
Leaf and stem micromorphology of Byrsonima sericea DC. by light and scanning electron microscopy
Anauara L E Silva1, Lucas E de Lima Terceiro2, Matheus F de Lima1
1Laboratório de Taxonomia e Farmacobotânica, Centro de Biotecnologia, Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil.
Abstract:
Micromorphological studies were carried out using multiple microscopic techniques on the leaves and stem bark of Byrsonima sericea DC. (Malpighiaceae), a species popularly known as "murici" and used medicinally, in order to identify both qualitative and quantitative features of leaf and stem anatomy and histochemistry as differential parameters to support both the quality control of its ethnodrugs and the taxonomy of the genus. The study was conducted using traditional techniques of plant anatomy, histochemical tests, and the stomatal index (SI). Byrsonima sericea has hypostomatic leaves, anomocytic stomata, and its epidermal walls are anticlinal and straight on the adaxial and curved on the abaxial faces. T-shaped trichomes were observed mainly on the abaxial surface. The leaf epidermis showed waxes syntopism on both surfaces, with the occurrence of different crystalloid forms on a single phylloplane. The mesophyll is dorsiventral, with 3-4 collateral vascular bundles. Phenolic compounds, starch, and proteins were identified in the petiole and stem. The SI was 14.5 ± 0.53% (p < .05), but did not showed significant variations. A set of characters were found to be distinctive for the studied species, however, constituting parameters that could be used to separate B. sericea from other species of the genus.
More Related Videos
Related Concept Videos
Microbial Morphologies
Overview of Electron Microscopy
Scanning Electron Microscopy
Fundamental Principles
Accelerated...

