Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.4K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.4K
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

4.4K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
4.4K
Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

1.4K
The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
1.4K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

644
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
644
Other Nuclides: 31P, 19F, 15N NMR01:16

Other Nuclides: 31P, 19F, 15N NMR

696
Many organic, inorganic, and biological molecules contain spin-half nuclei such as nitrogen-15, fluorine-19, and phosphorus-31. As a result, NMR studies of these nuclei have found extensive applications in chemical and biological research.
While fluorine-19 and phosphorous-31 have high natural abundances (100%) and positive gyromagnetic ratios, nitrogen-15 has a low natural abundance and a negative gyromagnetic ratio. However, nitrogen-15 is still preferred over nitrogen-14 (which has a...
696
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

577
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
577

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

'Getting control of Corona takes many angles': COVID-19 vaccine knowledge, attitudes and beliefs among refugee/immigrant/migrant communities in four US cities.

Health education research·2024
Same author

Water filtration by endobenthic sandprawns enhances resilience against eutrophication under experimental global change conditions.

Scientific reports·2023
Same author

Use of a sternocleidomastoid muscle flap to protect the carotid artery during laryngectomy.

The Journal of laryngology and otology·2021
Same author

Molecular characterization of plasmids encoding bla<sub>CTX-M</sub> from faecal Escherichia coli in travellers returning to the UK from South Asia.

The Journal of hospital infection·2021
Same author

Microbiology welcomes sequence papers.

Microbiology (Reading, England)·2021
Same author

Aqueous-Based Low-Temperature Synthesis and Thin-Film Properties of Oxysulfide BiCuOS Nanoparticles.

ChemPlusChem·2020
Same journal

Inhibiting selenium loss in sodium-selenium batteries <i>via</i> a Lewis acid-base dual-site host.

Chemical communications (Cambridge, England)·2026
Same journal

Switching cation selectivity <i>via</i> steric tuning in sumanene-based receptors.

Chemical communications (Cambridge, England)·2026
Same journal

Electrostatically polarized hydrogen-bonded organic frameworks for high-efficiency gold recovery from acidic electronic waste leachates.

Chemical communications (Cambridge, England)·2026
Same journal

Highly sensitive and wide-range non-contact fluorescent thermometry based on well-defined Cs<sub>2</sub>ZrCl<sub>6</sub>:Bi perovskite nanocrystals.

Chemical communications (Cambridge, England)·2026
Same journal

Effect of aluminum distribution in ZSM-22 zeolite on ethylene oligomerization.

Chemical communications (Cambridge, England)·2026
Same journal

Interfacial microenvironment engineering in CO<sub>2</sub> electroreduction: mechanisms, advances, and perspectives.

Chemical communications (Cambridge, England)·2026
See all related articles

Related Experiment Video

Updated: Jan 3, 2026

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
07:24

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

2.1K

Adding diffusion ordered NMR spectroscopy (DOSY) to the arsenal for characterizing paramagnetic complexes.

M P Crockett1, H Zhang, C M Thomas

  • 1Department of Chemistry, Boston College, Eugene F. Merkert Chemistry Center, 2609 Beacon Street, Chestnut Hill, Massachusetts 02467, USA. Jeffery.byers@bc.edu.

Chemical Communications (Cambridge, England)
|November 16, 2019
PubMed
Summary
This summary is machine-generated.

Diffusion Ordered NMR Spectroscopy (DOSY) now characterizes paramagnetic metal complexes. This technique assesses purity, speciation, and molecular weight for transition metal compounds.

More Related Videos

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.6K
15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the &#181;s-ms Timescale
08:09

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

5.9K

Related Experiment Videos

Last Updated: Jan 3, 2026

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
07:24

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

2.1K
Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.6K
15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the &#181;s-ms Timescale
08:09

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

5.9K

Area of Science:

  • Analytical Chemistry
  • Inorganic Chemistry
  • Nuclear Magnetic Resonance Spectroscopy

Background:

  • Characterizing metal complexes, especially those with paramagnetic transition metals, presents unique analytical challenges.
  • Traditional methods may struggle with purity assessment and speciation of these complex systems.

Purpose of the Study:

  • To develop and validate Diffusion Ordered NMR Spectroscopy (DOSY) for analyzing paramagnetic metal complexes.
  • To demonstrate DOSY's capability in assessing purity and speciation.
  • To explore DOSY for determining molecular weight data of paramagnetic complexes.

Main Methods:

  • Application of Diffusion Ordered NMR Spectroscopy (DOSY).
  • Characterization of first-row transition metal complexes.
  • Analysis of paramagnetic species using NMR.

Main Results:

  • Successfully adapted DOSY for paramagnetic metal complexes.
  • DOSY effectively assesses purity and speciation of these compounds.
  • Qualitative and sometimes quantitative molecular weight determination is achievable with DOSY.

Conclusions:

  • DOSY is a valuable technique for the characterization of paramagnetic metal complexes.
  • This method offers a convenient approach for purity, speciation, and molecular weight analysis.
  • Expands the utility of DOSY in inorganic and analytical chemistry.