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Rev1 wbdR tagged vaccines against Brucella ovis

Beatriz Aragón-Aranda1, María Jesús de Miguel2, Estrella Martínez-Gómez1

  • 1Instituto de Salud Tropical (ISTUN), Instituto de Investigación Sanitaria de Navarra (IdiSNA) and Dpto. de Microbiología y Parasitología, Universidad de Navarra, c/Irunlarrea 1, 31008, Pamplona, Spain.

Veterinary Research
|November 16, 2019
PubMed

Insights

Sheep brucellosis is a global concern. A modified Brucella melitensis Rev1 vaccine shows promise for protecting sheep against Ovis biovar, differentiating immune responses and aiding serodiagnosis.

Area of Science:

  • Veterinary immunology
  • Bacteriology
  • Vaccine development

Background:

  • Sheep brucellosis, caused by Brucella melitensis and B. ovis, presents a significant global challenge.
  • The existing B. melitensis Rev1 vaccine, while effective against B. melitensis, complicates serodiagnosis and is unsuitable for B. ovis eradication in Brucella melitensis-free regions.

Purpose of the Study:

  • To develop a modified Brucella melitensis Rev1 vaccine candidate for B. ovis.
  • To assess the immunogenicity and protective efficacy of the modified vaccine in a murine model.
  • To evaluate the serological differentiation capabilities of the modified vaccine.

Main Methods:

  • Genetic modification of B. melitensis Rev1 by deleting wbkC and incorporating wbdR.
  • Immunization of mice with the modified Rev1 strain.
  • Analysis of antibody responses and cross-reactivity.
  • Assessment of protection against B. ovis challenge.

Main Results:

  • The modified Rev1 vaccine elicited distinct antibody profiles compared to wild-type strains.
  • The vaccine candidate demonstrated significant attenuation in the murine model.
  • Mice vaccinated with the modified Rev1 showed protection against B. ovis challenge.
  • The induced antibodies allowed for differentiation from wild-type B. melitensis responses.

Conclusions:

  • A genetically modified Brucella melitensis Rev1 vaccine (Rev1 ΔwbkC/wbdR) is a potential candidate for a B. ovis vaccine.
  • This modified vaccine offers serological differentiation, overcoming limitations of the current Rev1 vaccine.
  • Further investigation is warranted to confirm its efficacy and safety in sheep for B. ovis control.

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