The functional characterization of peroxinectin in the defense of Fenneropenaeus penicillatus against pathogens

Peroxinectin with cell adhesion and peroxidase activities plays an important role in innate immune responses of invertebrate. In the study, the full-length cDNA of a peroxinectin homolog (FpPX) was identified from Fenneropenaeus penicillatus. The full-length cDNA of FpPX is 2573 bp long, with an open reading frame (ORF) encoding a putative protein of 780 amino acids, including a peroxidase domain and a KGD motif. FpPX shared 65-96% similarities with other crustaceans peroxinectin proteins. Real-time quantitative RT-PCR indicated that FpPX was constitutively expressed in gill, heart, hemocytes and muscle of F. penicillatus. The temporal expression patterns of FpPX mRNA were different in the various tissues after microbial challenge. FpPX expression levels were significantly upregulated in gill, hemocytes and muscle after white spot syndrome baculovirus (WSSV) or Vibrio alginolyticus injection. Conversely, FpPX expression in the heart maintained at a low level and showed no obvious changes at any of the tested time points. The results of RNAi experiment showed that silencing FpPX could inhibit prophenoloxidase expression in vivo, and lead to a significantly higher mortality of shrimps after WSSV or V. alginolyticus challenge, suggesting FpPX is required in defense against bacterial and viral pathogens. In conclusion, these data revealed that FpPX played an important role in immune response of F. penicillatus against pathogenic infection.