Effect of potassium on nitrate removal from groundwater in agricultural waste-based heterotrophic denitrification...

Heterotrophic denitrification based on solid carbon sources has been widely investigated for nitrogen removal in recent years. In this study, the response of the heterotrophic denitrification process under different K⁺ concentrations was clarified. Additionally, the denitrification enhancement mechanism was revealed and resource utilization of agricultural waste was achieved. A series of batch tests were conducted to study the effect of different K⁺ concentrations on the denitrification performance, dissolved organic matter (DOM) dissolution and microbial community structure. Results demonstrate that the threshold of K⁺ concentration for the NO₃^--N and NO₂^--N reduction rates were 229.78 ± 25.80 and 159.10 ± 24.60 mg-K/L, respectively. Excitation-emission matrix (EEM) analysis identified the main DOM components associated with tyrosine-like, tryptophan-like and humic-like substances, as well as illustrated the evolutionary behavior and utilization of DOM. High throughput 16S rRNA gene sequencing indicates that a K⁺ concentration of 229.78 ± 25.80 mg-K/L exhibited the highest diversity of functional species associated with fermentation and denitrification. The genera Pseudomonas and Thiobacillus were the unique denitrifiers at this K⁺ concentration. The correlation of K⁺ concentration, DOM dissolution of different regions and microorganism structure were analyzed using correlation matrix and PCA, and the appropriate K⁺ concentration of different functional microorganisms survival was optimized by this analysis method.