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Sensitively and Selectively Detect Biothiols by Using Fluorescence Method and Resonance Light Scattering Technique

Yanping Shi¹, Chao Sun¹, Xiaoqi Gao¹

¹Department of Chemistry, Northeast Agricultural University, Harbin 150025, China.

Molecules (Basel, Switzerland)

|November 17, 2019

Summary

This study introduces a novel dual-mode detection method for biothiols using nitrogen-doped carbon quantum dots and silver ions. The technique effectively distinguishes cysteine from glutathione in biological samples.

Keywords:

Ag+ complex IFE RLS technique biothiols carbon quantum dots

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Area of Science:

Analytical Chemistry
Biochemistry
Materials Science

Background:

Biothiols, such as cysteine (Cys) and glutathione (GSH), are crucial in biological processes.
Accurate and sensitive detection of biothiols is essential for diagnosing and monitoring various diseases.
Existing detection methods often face challenges in distinguishing between different biothiols.

Purpose of the Study:

To develop a novel, dual-mode quantitative and qualitative detection method for biothiols.
To utilize nitrogen-doped carbon quantum dots (C/N-dots) and silver ions (Ag+) for enhanced detection capabilities.
To differentiate between cysteine and glutathione in complex biological matrices.

Main Methods:

Synthesis of C/N-dots from tartaric acid and ethylenediamine via hydrothermal method.
Establishment of a detection system combining the photoluminescent property of C/N-dots and the resonance light scattering (RLS) property of Ag+ complex.
Optimization of detection conditions and investigation of fluorescence quenching and RLS mechanisms.

Main Results:

The fluorescence method achieved a linear response for Cys in the range of 0-140 μM with a detection limit (LOD) of 6.6 μM, unaffected by GSH.
The RLS technique provided linear responses for Cys (0-167 μM) and GSH (0-200 μM) with LODs of 64 nM and 74 nM, respectively.
The developed probe was successfully applied to detect Cys in fetal bovine serum (FBS) using fluorescence and GSH in FBS using RLS.

Conclusions:

The C/N-dots and Ag+ complex system offers a sensitive and selective dual-mode platform for biothiol detection.
This method enables the simultaneous quantitative and qualitative analysis of Cys and GSH, overcoming limitations of single-mode detection.
The probe's successful application in real biological samples demonstrates its potential for practical diagnostic applications.