Lupine protein hydrolysates decrease the inflammatory response and improve the oxidative status in human peripheral...

Area of Science:

• Immunology
• Nutritional Science
• Oxidative Stress Research

Background:

• Previous studies on lupine proteins used cell-free systems or immortalized cells.
• The effects of lupine protein hydrolysates (LPHs) on non-immortalized human immune cells remained unexamined.
• Understanding LPHs' impact on human peripheral blood mononuclear cells (PBMCs) is crucial for nutritional applications.

Purpose of the Study:

• To investigate the in vitro effects of LPHs from Lupinus angustifolius on human PBMCs.
• To assess the impact of LPHs on immunological and oxidative stress markers in non-immortalized human cells.

Main Methods:

• Human peripheral blood mononuclear cells (PBMCs) were isolated from 53 healthy donors.
• PBMCs were treated in vitro with LPHs from Lupinus angustifolius.
• Assessed PBMCs proliferation, pro-inflammatory cytokine levels (Th1, Th9, Th17), antioxidant enzyme activities (superoxide dismutase, catalase), and total antioxidant capacity (TAC).

Main Results:

• LPHs significantly reduced PBMCs proliferation and levels of pro-inflammatory cytokines (Th1, Th9, Th17) without cytotoxicity.
• LPHs shifted the cytokine balance towards a protective Th2 response.
• LPHs enhanced superoxide dismutase and catalase activities, increasing total antioxidant capacity (TAC).

Conclusions:

• This study demonstrates for the first time that LPHs modulate T cell inflammatory responses in human PBMCs.
• LPHs improve the pro-inflammatory/anti-inflammatory cytokine balance and enhance the antioxidant status of PBMCs.
• LPHs show promise as a component of nutritional strategies for preventing diseases linked to inflammation and oxidative stress.