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lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

Transducer Mechanism: Nuclear Receptors

Transducer Mechanism: Nuclear Receptors

Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:

Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

Types of RNA

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

Regulation of Nuclear Protein Sorting

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

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Related Experiment Video

Updated: Jan 3, 2026

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Published on: March 1, 2019

LncNONO-AS regulates AR expression by mediating NONO.

Zhongquan Zhao¹, Lei Qiao¹, Zinuo Dai¹

¹College of Animal Science and Technology, Southwest University, Chongqing, 400715, China.

|November 17, 2019

Summary

A novel long non-coding RNA, lncNONO-AS, was identified in goat testes and found to regulate androgen receptor (AR) expression by mediating NONO. This discovery sheds light on male infertility mechanisms.

Keywords:

Androgen receptor Antisense lncRNA Goat’s testicle NONO Spermatogenesis

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Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

Published on: November 15, 2013

Area of Science:

Reproductive Biology
Molecular Endocrinology
Genomics

Background:

Spermatogenesis and testicular development are crucial for male fertility.
Androgen receptor (AR) dysfunction in Sertoli cells impairs sperm development and causes infertility.
Long non-coding RNAs (lncRNAs) play vital roles in spermatogenesis, but their specific functions in goat testes are underexplored.

Purpose of the Study:

To identify and characterize a novel lncRNA involved in NONO-AR regulation in goat testicular Sertoli cells.
To investigate the functional role of this lncRNA in male reproductive processes.

Main Methods:

High-throughput sequencing and bioinformatics analysis of Dazu black goat testicular tissues.
In vitro culture of goat testis Sertoli cells.
RNA pull down, overexpression, interference, fluorescence quantification, and Western blot assays.

Main Results:

A new lncRNA, named lncNONO-AS, was identified and found to target NONO.
Overexpression of lncNONO-AS significantly increased NONO and AR gene expression.
Interference with lncNONO-AS led to decreased expression of NONO and AR.

Conclusions:

lncNONO-AS acts as a regulator of AR expression by mediating NONO in goat testicular Sertoli cells.
This finding provides new insights into the molecular mechanisms underlying male reproductive function and infertility.
lncNONO-AS represents a potential target for addressing male infertility issues.