Early antibody responses map to non-protective, PCV2 capsid protein epitopes

Porcine circovirus type 2 (PCV2) is an economically important cause of post-weaning multisystemic wasting syndrome (PMWS) in weanling piglets. Current commercial vaccines against PCV2 are highly effective. Yet, a recurring emergence of new genotypes in vaccinated herds necessitates a better understanding of protective immunity. The study objectives were to identify previously unrecognized decoy epitopes in the PCV2 capsid and test the hypothesis that early antibody responses would map to decoy epitopes and vice versa. Using a peptide library spanning the PCV2a capsid and weekly sera collections from PCV2a infected animals, three major immunodominant regions mapping the early responses to decoy epitopes were identified. Regions with potential decoy activity were mapped using peptide blocking fluorescent focus inhibition assays to residues 55 YTVKATTVRTPSWAVDMM 72, 106 WPCSPITQGDRGVGSTAV 123 and 124 ILDDNFVTKATALTYDPY 141. Post-vaccination responses largely recognized these same three identified regions and dominated the antibody responses to PCV2 in both infection and vaccination.