Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

1.6K
A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
1.6K
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

577
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
577
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

644
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
644
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

706
Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
706
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

2.0K
NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
2.0K
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

1.0K
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
1.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<sup>15</sup>N-filtered, <sup>13</sup>C-detected spin-correlations in solid-state NMR of macroscopically oriented samples.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same author

Integration of alternative fragmentation techniques into standard LC-MS workflows using a single deep learning model enhances proteome coverage.

Nature methods·2026
Same author

Prosit-XL: enhanced cross-linked peptide identification by fragment intensity prediction to study protein interactions and structures.

Nature communications·2025
Same author

Integrating Alternative Fragmentation Techniques into Standard LC-MS Workflows Using a Single Deep Learning Model Enhances Proteome Coverage.

bioRxiv : the preprint server for biology·2025
Same author

Pairwise Attention: Leveraging Mass Differences to Enhance De Novo Sequencing of Mass Spectra.

Journal of proteome research·2025
Same author

Room-Temperature Pulsed Dynamic Nuclear Polarization at 7 T.

The journal of physical chemistry letters·2025

Related Experiment Video

Updated: Jan 3, 2026

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the &#181;s-ms Timescale
08:09

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

5.9K

De novo NMR pulse sequence design using Monte-Carlo optimization techniques.

Joel Lapin1, Alexander A Nevzorov1

  • 1Department of Chemistry, North Carolina State University, 2620 Yarbrough Drive, Raleigh, NC 27695-8204, United States.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|November 18, 2019
PubMed
Summary
This summary is machine-generated.

Researchers developed novel Separated Local Field (SLF) pulse sequences for solid-state NMR, significantly improving protein structure determination by sharpening spectral linewidths. The new ROULETTE-1 sequence offers superior performance compared to existing methods.

Keywords:
AutomationDipolar couplingsGPU computingNMR pulse sequence designPISEMASAMPI4Separated local field experimentsSimulated annealing

More Related Videos

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.6K
Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
07:24

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

2.1K

Related Experiment Videos

Last Updated: Jan 3, 2026

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the &#181;s-ms Timescale
08:09

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

5.9K
Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.6K
Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
07:24

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

2.1K

Area of Science:

  • Solid-state Nuclear Magnetic Resonance (NMR) spectroscopy
  • Protein structure determination
  • Computational chemistry and biophysics

Background:

  • Separated Local Field (SLF) experiments are crucial for measuring 1H-15N heteronuclear dipolar couplings in oriented solid-state NMR.
  • Traditional analytical methods for optimizing SLF pulse sequences become complex with increasing parameters.
  • Numerical simulations offer a viable alternative for rapid spectral calculations and pulse sequence optimization.

Purpose of the Study:

  • To develop an efficient computational strategy for optimizing SLF pulse sequences.
  • To enhance 1H-15N dipolar linewidths for improved protein structure determination.
  • To discover novel pulse sequences outperforming existing methods like SAMPI4.

Main Methods:

  • Utilized GPU-accelerated numerical simulations for rapid spectral calculations.
  • Employed Monte Carlo Simulated Annealing for efficient numeric optimization of pulse sequences.
  • Developed and tested over 100 de novo pulse sequences on an N-acetyl Leucine crystal.

Main Results:

  • Seventeen novel pulse sequences demonstrated sharper mean linewidths than SAMPI4.
  • The top-performing sequence, ROULETTE-1, achieved 18% sharper mean linewidths on N-acetyl Leucine.
  • ROULETTE-1 showed robustness across various 1H carrier frequencies and crystal orientations, and improved signal-to-noise ratio on Pf1 coat protein.

Conclusions:

  • A computational strategy using numerical simulations and simulated annealing effectively optimizes SLF pulse sequences.
  • The developed ROULETTE-1 sequence significantly improves 1H-15N dipolar linewidths and signal-to-noise ratio in solid-state NMR.
  • This approach is extendable for the de novo development of various NMR experiments.