Detection of Fungal Jasmonates by Liquid Chromatography Paired with Mass Spectrometry
Yingyao Liu1, Rajesh N Patkar2, Naweed I Naqvi3
1Temasek Life Sciences Laboratory, Singapore, Singapore.
Abstract:
Liquid chromatography-mass spectrometry (LC-MS) is one of the most important analytical chemistry techniques for the detection and characterization of biologically active compounds of low abundance-for example, hormones. Gas chromatography (GC) coupled with mass spectrometry has been a method of choice to detect jasmonic acid, the well-known defense hormone in plants. Recently, we identified structural and functional analogs of phytohormone jasmonic acid (JA) and its derivatives, in the rice-blast fungus Magnaporthe oryzae. Here, we describe protocols of LC-MS/MS-based identification and quantification of fungal jasmonates, especially during pathogenic development in the rice blast fungus.
More Related Videos
11:14Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry
Published on: October 2, 2016
09:36Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques
Published on: March 8, 2024
Related Concept Videos
MALDI-TOF Mass Spectrometry
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
High-Performance Liquid Chromatography: Types of Detectors
Tandem Mass Spectrometry
Mass Spectrometry: Aromatic Compound Fragmentation
