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Published on: August 7, 2018
DNase γ-dependent DNA fragmentation causes karyolysis in necrotic hepatocyte
Shuhei Takada1, Taiki Watanabe1, Ryushin Mizuta1
1Research Institute for Biomedical Sciences, Tokyo University of Science, Noda, Chiba 278-0022, Japan.
Abstract:
Karyolysis is the complete dissolution of nuclear components of a dying cell. However, the generation mechanism has not been clarified. We studied a necrotic DNA fragmentation factor DNase γ (also known as DNase1L3) and previously found that karyolysis was inhibited in DNase γ deficient (DNase γ-/-) mice. To confirm this, we transiently expressed DNase γ in the liver of DNase γ-/- mice and caused hepatocyte necrosis by acetaminophen overdose. As expected, karyolysis was induced in the necrotic hepatocytes. We also found that the depletion of Kupffer cells from wild type mice reduced the expression and activity of DNase γ in the liver. Thus, we concluded that DNase γ produced from Kupffer cells caused karyolysis of necrotic hepatocytes.
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