Related Experiment Video

Updated: Jan 3, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.3K

Contribution of alternative splicing to breast cancer metastasis

Xiangbing Meng1,2, Shujie Yang1,2, Jun Zhang2,3

  • 1Department of Obstetrics and Gynecology, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USA.

Journal of Cancer Metastasis and Treatment
|November 19, 2019
PubMed

Insights

Alternative splicing significantly impacts cancer, with more events found in tumors. This review explores its role in breast cancer metastasis, focusing on MTDH and CD44.

Area of Science:

  • Molecular Biology
  • Genomics
  • Cancer Research

Background:

  • Alternative splicing generates transcriptome and proteome diversity in eukaryotes.
  • Approximately 30% more alternative splicing events are observed in 32 cancer types compared to normal samples.
  • Specific alternative splicing isoforms and their proteins contribute to cancer hallmarks.

Purpose of the Study:

  • To review recent advancements in understanding alternative splicing's contribution to breast cancer metastasis.
  • To dissect the roles of MTDH and CD44 in alternative splicing related to breast cancer metastasis.
  • To explore the interaction between MTDH, CD44, and other mRNA splicing factors.

Main Methods:

  • Literature review of recent progress in alternative splicing and breast cancer metastasis.
  • Analysis of data from The Cancer Genome Atlas (TCGA) database.
  • Focus on specific genes like MTDH and CD44 and their splicing factors.

Main Results:

  • Alternative splicing is significantly altered in various cancers, including breast cancer.
  • Certain splicing events and isoforms are linked to the development and progression of breast cancer metastasis.
  • MTDH and CD44 are identified as key players in splicing-driven metastasis.

Conclusions:

  • Alternative splicing plays a critical role in breast cancer metastasis.
  • Understanding the mechanisms of splicing in metastasis can lead to new therapeutic strategies.
  • Targeting splicing factors or isoforms may offer novel approaches for breast cancer management.
Keywords:
Breast cancerCD44MTDHepithelial-mesenchymal transitionmetastasissplicing

More Related Videos

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

13.3K
Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts11:19

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Published on: October 9, 2016

15.4K

Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.2K
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
24.5K
Alternative RNA Splicing02:18

Alternative RNA Splicing

4.7K
Metastasis02:30

Metastasis

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
6.3K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
8.0K
Cancers Originate from Somatic Mutations in a Single Cell02:21

Cancers Originate from Somatic Mutations in a Single Cell

Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
14.5K