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Dynamic host-guest interaction enables autonomous single molecule blinking and super-resolution imaging.

Ranjan Sasmal1, Nilanjana Das Saha, Florian Schueder

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We developed a new method for super-resolution imaging using dynamic host-guest chemistry. This technique allows for stochastic switching of fluorescence, enabling nanoscale imaging within cells and tissues.

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Area of Science:

  • Supramolecular Chemistry
  • Chemical Biology
  • Nanotechnology

Background:

  • Synthetic host-guest complexes rely on dynamic, reversible noncovalent interactions.
  • Understanding and controlling these dynamic interactions is key for advanced molecular applications.

Purpose of the Study:

  • To exploit dynamic supramolecular recognition for developing novel imaging techniques.
  • To achieve stochastic fluorescence switching for super-resolution imaging in biological systems.

Main Methods:

  • Utilized fluorescently labeled guest molecules and cucurbit[7]uril hosts for dynamic supramolecular recognition.
  • Engineered stochastic switching between fluorescence ON- and OFF-states.
  • Applied the method for PAINT-based (pristine area-invariant nanoscopic tracking) nanoscopic imaging.

Main Results:

  • Demonstrated successful stochastic fluorescence switching through host-guest complexation.
  • Enabled high-resolution PAINT imaging in cellular and tissue environments.
  • Showcased the dynamic nature of synthetic host-guest complexes for advanced applications.

Conclusions:

  • Dynamic supramolecular recognition offers a powerful strategy for stochastic switching in molecular imaging.
  • PAINT-based nanoscopic imaging in cells and tissues is achievable using engineered host-guest systems.
  • This work advances the application of supramolecular chemistry in biological imaging.