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Updated: Jan 3, 2026
Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
Published on: July 4, 2011
A NIR Turn-on Fluorescent Sensor For Detection of Chloride Ions in vitro and in vivo
Fengyuan Zhang1, Chen Ma2, Zhijuan Jiao2
1State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China; School of Basic Medical Science, Ningxia Medical University, Yinchuan, 750004, China.
Abstract:
As the most abundant and significant anions in biosystem, chloride ions (Cl-) participate in many important physiological processes. Thus, designing and synthesizing of a simple, sensitive, selective and long wavelength turn-on sensor for the detection and imaging of Cl- in vitro and in vivo is very necessary. Herein, we have developed a simple porphyrin turn-on sensor 5, 10, 15, 20-Tetrakis (4-hydroxyphenyl) porphyrin (THPP) with near infrared emission wavelength (657 nm) for sensing chloride ions with remarkable sensitivity and selectivity. The detection of chloride ions was according to metal displacement assay (MDA) under physiological condition with a detection limit of 7.5 μM, and was applied to image Cl- in vitro and in vivo successfully.
