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Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
Published on: June 21, 2018
Precision Medicine and Mysteries in Clinical Microbiology: Rationalizing Epidemiology, Genotype, and Phenotype To
Federico Perez1,2,3, Robert A Bonomo1,2,4,5,6,7,3
1Medical and Research Services and Geriatrics Research, Education and Clinical Center (GRECC), VA Northeast Ohio Healthcare System and Louis Stokes Cleveland VA Medical Center, Cleveland, Ohio, USA federico.perez@va.gov robert.bonomo@va.gov.
Abstract:
Whole-genome sequencing (WGS) using MinION was used to characterize high-risk clones of Escherichia coli and Klebsiella pneumoniae harboring bla NDM-5, bla OXA-181, and bla CTX-M-15, as well as Pseudomonas aeruginosa harboring bla NDM, in a patient who received health care in India. Synergy testing demonstrated the activity of aztreonam and ceftazime-avibactam in combination. This case illustrates a "precision medicine" approach where deeper understanding of the genotype through WGS and of the phenotype through synergy testing formed the basis for rational combination therapy.
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