Jove
Visualize
Contact Us

Related Concept Videos

Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

487
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
487
Riboswitches01:56

Riboswitches

9.5K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
9.5K
Translational Regulation01:29

Translational Regulation

484
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
484
Regulated mRNA Transport02:22

Regulated mRNA Transport

6.8K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
6.8K
Regulated mRNA Transport02:22

Regulated mRNA Transport

3.3K
3.3K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.3K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aircraft observations of black carbon over the Yellow Sea and Seoul Metropolitan Area: Vertical profiles and air mass origin influence.

Journal of environmental sciences (China)·2026
Same author

Reduced MOV10 reveals novel functional cortical connections in an increased fear response.

bioRxiv : the preprint server for biology·2026
Same author

Phase 1 evaluation of patients with newly diagnosed glioblastoma treated with radiation, nivolumab, and IDO1 enzyme inhibitor BMS-986205.

Clinical cancer research : an official journal of the American Association for Cancer Research·2026
Same author

Factors Predicting Guselkumab Treatment Response in Patients with Moderate-to-Severe Plaque Psoriasis: A Post Hoc Analysis of Korean Real-World Data.

Journal of clinical medicine·2026
Same author

Are climate change perceptions related with plastic policy support? Effects of climate change skepticism, guilt, and efficacy on the acceptance of the plastic tax.

PloS one·2025
Same author

Greenhouse-Gas-Driven Room-Temperature Synthesis of Compositionally Complex Nanomaterials via Anion-Cation Arrangement Control.

Nano letters·2025
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jan 3, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.1K

The FMRP-MOV10 complex: a translational regulatory switch modulated by G-Quadruplexes.

Phillip J Kenny1, Miri Kim2, Geena Skariah2

  • 1Cell and Developmental Biology, University of Illinois-Urbana Champaign, Urbana, IL 61801, USA.

Nucleic Acids Research
|November 20, 2019
PubMed
Summary

Fragile X Mental Retardation Protein (FMRP) regulates microRNA (miRNA) silencing via MOV10 and G-quadruplexes. FMRP

More Related Videos

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.5K
Enhanced Crosslinking Immunoprecipitation eCLIP Method for Efficient Identification of Protein-bound RNA in Mouse Testis
10:31

Enhanced Crosslinking Immunoprecipitation eCLIP Method for Efficient Identification of Protein-bound RNA in Mouse Testis

Published on: May 10, 2019

20.6K

Related Experiment Videos

Last Updated: Jan 3, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.1K
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.5K
Enhanced Crosslinking Immunoprecipitation eCLIP Method for Efficient Identification of Protein-bound RNA in Mouse Testis
10:31

Enhanced Crosslinking Immunoprecipitation eCLIP Method for Efficient Identification of Protein-bound RNA in Mouse Testis

Published on: May 10, 2019

20.6K

Area of Science:

  • Molecular Biology
  • Neuroscience
  • Genetics

Background:

  • Fragile X Mental Retardation Protein (FMRP) is crucial for normal cognition and regulates mRNA translation.
  • FMRP interacts with RNA helicase Moloney leukemia virus 10 (MOV10) to modulate microRNA (miRNA)-mediated silencing.
  • G-quadruplex structures play a role in FMRP's bi-functional regulatory activities.

Purpose of the Study:

  • To elucidate the mechanism by which FMRP suppresses Argonaute (AGO) association with mRNAs.
  • To identify the specific domains of FMRP, MOV10, and AGO involved in their interactions.
  • To understand the role of MOV10's N-terminus in FMRP-mediated translational regulation and neurite outgrowth.

Main Methods:

  • Domain mapping of FMRP, MOV10, and AGO interactions.
  • Analysis of mRNA and protein levels following MOV10 overexpression.
  • Investigation of FMRP's role in recruiting AGO2 to RNAs in the mouse brain.

Main Results:

  • The RGG box of FMRP protects specific mRNAs from AGO association.
  • The N-terminus of MOV10 is essential for this protective mechanism and promotes neurite outgrowth.
  • FMRP globally influences miRNA-mediated translation by recruiting AGO2 to a significant subset of RNAs in the mouse brain.

Conclusions:

  • FMRP, through its RGG box and interaction with MOV10, precisely controls miRNA-mediated translational silencing.
  • MOV10's N-terminus is critical for FMRP's function in protecting mRNAs and promoting neuronal development.
  • These findings reveal a global role for FMRP in miRNA-mediated translational regulation essential for cognitive function.