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Stable transformation of unicellular green alga Coccomyxa subellipsoidea C-169 via electroporation
Kinga Kania1, Maksymilian Zienkiewicz1, Anna Drożak2
1Department of Molecular Plant Physiology, University of Warsaw, Warsaw, Poland.
Protoplasma
|November 20, 2019
Abstract:
In this study, we have shown the applicability of electroporation and hygromycin B as a convenient selectable marker for stable nuclear transformation of Coccomyxa subellipsoidea C-169. Since it is the first sequenced eukaryotic microorganism from polar environment, this offers unique opportunities to study adaptation mechanisms to cold.

