Autonomous Temporal Control of Hydrogen Sulfide Delivery
Maheshwar Adiraj Iyer1, David Eddington1
1Department of Bioengineering , University of Illinois at Chicago , Chicago , Illinois 60607 , United States.
Analytical Chemistry
|November 20, 2019
Summary
Hydrogen sulfide (H2S) is a gasotransmitter with dual effects. This study introduces a novel feedback-controlled microfluidic system for precise, stable H2S delivery in experiments, overcoming challenges with toxic gas handling and donor decay.
Area of Science:
- Biochemistry
- Physiology
- Microfluidics
Background:
- Hydrogen sulfide (H2S) acts as a gasotransmitter, influencing physiological processes.
- H2S exhibits dichotomous effects, being cytotoxic at high concentrations and cytoprotective at low concentrations.
- Accurate experimental models for H2S are crucial but challenging due to its toxicity and unstable chemical donors.
Purpose of the Study:
- To develop a novel experimental method for precise hydrogen sulfide (H2S) delivery.
- To address the limitations of existing methods, including toxic gas handling and temporally varying release profiles from chemical donors.
- To establish a stable and controllable in vitro environment for studying H2S.
Main Methods:
- Utilized microfluidics to create a stable H2S delivery system.
- Incorporated a feedback control mechanism to precisely regulate H2S concentrations.
- Accounted for the decay of donor solution concentration over time.
Main Results:
- Demonstrated a novel experimental method for precise H2S delivery.
- Achieved stable H2S concentrations, overcoming donor solution decay issues.
- Enabled controlled H2S exposure in long-term cell cultures.
Conclusions:
- The developed feedback-controlled microfluidic system offers precise and stable H2S delivery.
- This method overcomes significant challenges in experimental H2S research.
- Facilitates the generation of reliable in vitro and in vivo models for studying H2S gasotransmission.
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