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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Meroterpenoids and alkaloids from Ganoderma australe
Jiao-Jiao Zhang1,2, Yun Dong1,3, Fu-Ying Qin1,2
1School of Pharmaceutical Sciences, Shenzhen University Health Science Center, Shenzhen, PR China.
Two new compounds from Ganoderma australe, australins A and B, and alkaloid australine were identified. Certain compounds showed neuroprotective effects against excitotoxicity in cell models.
Area of Science:
- Natural Product Chemistry
- Pharmacology
- Mycology
Background:
- Ganoderma species are known sources of bioactive compounds.
- Neurodegenerative diseases pose a significant health burden.
- Understanding natural compounds' neuroprotective potential is crucial.
Purpose of the Study:
- To isolate and characterize novel compounds from Ganoderma australe.
- To evaluate the neuroprotective effects of isolated compounds.
Main Methods:
- Isolation and structure elucidation using spectroscopic methods (NMR, MS) and ECD calculations.
- Chiral High-Performance Liquid Chromatography (HPLC) for racemic compound separation.
- In vitro cell-based assays using SH-SY5Y cells to assess neuroprotection against glutamate excitotoxicity.
Main Results:
- Two new meroterpenoids (australins A and B) and a new alkaloid (australine) were identified.
- Five known compounds were also isolated and characterized.
- Compounds (+)-australin A and a known compound (7) demonstrated significant protection of SH-SY5Y cells from glutamate-induced excitotoxicity.
Conclusions:
- Ganoderma australe is a source of novel meroterpenoids and alkaloids.
- The identified compounds (+)-australin A and compound 7 possess neuroprotective properties.
- These findings suggest potential therapeutic applications for neurodegenerative diseases.
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