Related Experiment Video
Updated: Jan 3, 2026

Recognition of Epidermal Transglutaminase by IgA and Tissue Transglutaminase 2 Antibodies in a Rare Case of Rhesus Dermatitis
Published on: December 15, 2011
Trypsin cleavage sites are highly unlikely to occur in celiac-causing restricted epitopes
Rod A Herman1, Ping Song1, Henry P Mirsky2
1Regulatory and Stewardship, CortevaTM Agriscience, Indianapolis, IN, USA.
Abstract:
To assess risk, the European Food Safety Authority requires that the amino-acid sequences of newly expressed proteins in genetically engineered (GE) crops should be searched for partial matches with 9-mer restricted epitopes known to cause celiac disease. None of the 26 known celiac-causing 9-mer epitopes contain an in-silico predicted trypsin cleavage site. The probability of this occurring by chance alone is 0.000056. Based on the absence of in-silico predicted trypsin cleavage sites within 9-mer epitopes known to cause celiac disease, it can be concluded with very high confidence that true celiac-causing epitopes are highly unlikely to contain in-silico predicted trypsin cleavage sites and that this criterion can reliably be used to exclude the risk that imperfect 9-mer peptide matches within newly expressed proteins from GE crops cause celiac disease.
More Related Videos
11:25Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
Published on: October 4, 2017
08:56Demonstration of Proteolytic Activation of the Epithelial Sodium Channel ENaC by Combining Current Measurements with Detection of Cleavage Fragments
Published on: July 5, 2014
Related Concept Videos
Protein Digestion
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
Protein Absorption
Cross-reactivity
Restriction Enzymes
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
Protein Folding Quality Check in the RER