Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spiro‑Linked Double Tetraphenylethenes: Solid-State Emission and Circularly Polarized Luminescence Imaging.

Angewandte Chemie (International ed. in English)·2026
Same author

Protein-Directed Nucleation and Stabilization of Ultrasmall Silver Nanoparticles Within BSA Hydrogels.

Gels (Basel, Switzerland)·2026
Same author

Stereospecific Assembly of Triply Chiral Pseudopeptidic Cages Through Dynamic Dual Chirality Transfer.

Angewandte Chemie (International ed. in English)·2026
Same author

Liposomal formulation of the CDK9 PROTAC THAL-SNS-032 enhances the antitumor activity in breast cancer cell lines.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2025
Same author

Gamma-Glutamyl Cysteine Ligase Activity as a Proxy for Human T Cell Function and Drug-Induced Immunosuppression.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Protein aggregation monitoring in cells under oxidative stress: a novel fluorescent probe based on a 7-azaindole-BODIPY derivative.

Journal of materials chemistry. B·2024
Same journal

RETRACTED: Zhang et al. A Novel Framework for Reconstruction and Imaging of Target Scattering Centers via Wide-Angle Incidence in Radar Networks. <i>Sensors</i> 2025, <i>25</i>, 6802.

Sensors (Basel, Switzerland)·2026
Same journal

Enhancing Unsupervised Multi-Source Domain Adaptation for Person Re-Identification via Mixture of Experts and Graph-Based Relation.

Sensors (Basel, Switzerland)·2026
Same journal

Development of an Instrumented Glove for Palmar Pressure Assessment in Kayakers.

Sensors (Basel, Switzerland)·2026
Same journal

Development and Experimental Validation of an Autonomous IoT-Based Monitoring System for Real-Time Water Quality Assessment in the Amazon River.

Sensors (Basel, Switzerland)·2026
Same journal

Semi-Supervised Adversarial Learning Framework for Controller Area Network Bus Intrusion Detection.

Sensors (Basel, Switzerland)·2026
Same journal

Smart Optimization Method for Safety Signs in Innovative Manufacturing Environments Integrating Industrial Field IoT Sensors and Knowledge Graphs.

Sensors (Basel, Switzerland)·2026
See all related articles

Related Experiment Video

Updated: Jan 3, 2026

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

2.0K

A Quantum Dot-Based FLIM Glucose Nanosensor.

Consuelo Ripoll1, Angel Orte1, Lorena Paniza1

  • 1Department Physical Chemistry, Faculty of Pharmacy, Unidad de Excelencia de Química Aplicada a la Biomedicinay Medioambiente (UEQABM), University of Granada, Campus Cartuja, 18071 Granada, Spain.

Sensors (Basel, Switzerland)
|November 21, 2019
PubMed
Summary
This summary is machine-generated.

Quantum dots (QDs) modified with aminophenylboronic acid (APBA) function as glucose nanosensors. These nanosensors utilize fluorescence lifetime imaging microscopy (FLIM) for intracellular glucose detection, offering improved selectivity over traditional methods.

Keywords:
fluorescence lifetime imagingglucoseintracellular sensingnanoparticlesphotoluminescencequantum dots

More Related Videos

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

18.6K
Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing
08:12

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

13.1K

Related Experiment Videos

Last Updated: Jan 3, 2026

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

2.0K
Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

18.6K
Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing
08:12

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

13.1K

Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Analytical Chemistry

Background:

  • Quantum dots (QDs) offer excellent optical properties for bioimaging and sensing.
  • Photoluminescence (PL) intensity-based methods face limitations in intracellular environments, including signal fluctuations and autofluorescence interference.
  • Time-resolved spectroscopy and fluorescence lifetime imaging microscopy (FLIM) offer solutions to overcome these limitations.

Purpose of the Study:

  • To develop novel QD-APBA conjugates as nanosensors for glucose detection.
  • To utilize FLIM for sensitive and selective intracellular glucose monitoring.
  • To explore the potential of these nanosensors in cancer diagnosis.

Main Methods:

  • Synthesis of CdSe/ZnS QDs modified with aminophenylboronic acid (APBA).
  • Characterization of QD-APBA conjugates using time-resolved spectroscopy.
  • Application of QD-APBA nanosensors for intracellular glucose detection in MDA-MB-231 cells using FLIM.

Main Results:

  • Attachment of APBA to QDs resulted in quenched PL average lifetime.
  • Glucose binding to APBA-QDs led to recovery and enhancement of PL lifetime.
  • Successful detection of intracellular glucose in cancer cells using FLIM.
  • QD nanoparticles with long PL lifetimes were distinguishable from cell autofluorescence, enhancing sensing selectivity.

Conclusions:

  • QD-APBA conjugates serve as effective nanosensors for intracellular glucose detection.
  • FLIM-based sensing with long-lifetime QDs improves selectivity by minimizing autofluorescence interference.
  • The developed nanosensors show potential for cancer diagnosis due to the link between intracellular glucose levels and cancer cell metabolism.