Related Experiment Video

Updated: Jan 3, 2026

In Vitro Model of Coronary Angiogenesis
08:03

In Vitro Model of Coronary Angiogenesis

Published on: March 10, 2020

8.4K

Genetic variants of VEGFR-1 gene promoter in acute myocardial infarction

Haihua Wang1, Shufang Zhang2, Na Wang3

  • 1Shandong Provincial Key Laboratory of Cardiac Disease Diagnosis and Treatment, Affiliated Hospital of Jining Medical University, Jining Medical University, 89 Guhuai Road, Jining, 272029, Shandong, China.

Human Genomics
|November 21, 2019
PubMed

Insights

Genetic variants in the Vascular Epithelial Growth Factor Receptor-1 (VEGFR-1) gene promoter were identified in patients with Acute Myocardial Infarction (AMI). These variants may impact VEGFR-1 gene activity, potentially contributing to AMI development.

Area of Science:

  • Cardiovascular Genetics
  • Molecular Biology
  • Disease Pathogenesis

Background:

  • Coronary artery disease (CAD), including acute myocardial infarction (AMI), is a complex condition driven by atherosclerosis.
  • Vascular Epithelial Growth Factor Receptor-1 (VEGFR-1) plays a role in angiogenesis and vascular permeability, and its absence leads to cardiac issues in mice.
  • VEGFR-1 gene variants are hypothesized to be involved in CAD due to observed cardiac dysfunction and fibrosis in related studies.

Purpose of the Study:

  • To investigate the potential role of VEGFR-1 gene variants in the development of AMI.
  • To genetically and functionally analyze the VEGFR-1 gene promoter in large cohorts of AMI patients and controls.

Main Methods:

  • Identification of DNA sequence variants (DSVs) and single-nucleotide polymorphisms (SNPs) in the VEGFR-1 gene promoter and 5'-untranslated region.
  • Functional analysis of identified variants using reporter gene assays in HEK-293 and H9c2 cells to assess transcriptional activity.
  • Electrophoretic mobility shift assays (EMSA) to evaluate the impact of variants on transcription factor binding.

Main Results:

  • Sixteen DNA sequence variants (DSVs), including six single-nucleotide polymorphisms (SNPs), were identified in the VEGFR-1 gene promoter and 5'-untranslated region.
  • Five novel DSVs and one SNP were exclusively found in the AMI patient group.
  • These variants significantly altered VEGFR-1 gene promoter transcriptional activity and affected transcription factor binding.

Conclusions:

  • Genetic variants within the VEGFR-1 gene promoter identified in AMI patients may influence its transcriptional activity.
  • These alterations in transcriptional activity could lead to changes in VEGFR-1 levels, potentially contributing to the pathogenesis of AMI.
Abstract

Keywords:
Acute myocardial infarctionDNA sequence variantsGeneticsPromoterVEGFR-1

More Related Videos

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease04:41

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Published on: July 3, 2018

8.6K
Gene Transfer for Ischemic Heart Failure in a Preclinical Model07:35

Gene Transfer for Ischemic Heart Failure in a Preclinical Model

Published on: May 15, 2011

13.3K

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.3K
Acute Coronary Syndrome III: Diagnostic Studies01:30

Acute Coronary Syndrome III: Diagnostic Studies

Diagnosing acute coronary syndrome or ACS begins with a thorough patient history. Notable symptoms include central, crushing chest pain radiating to the left arm, neck, jaw, or back, along with shortness of breath, sweating (diaphoresis), nausea, vomiting, dizziness, and palpitations.It is crucial to note any history of cardiac illnesses and assess risk factors, including age, gender, smoking, hypertension, diabetes, hyperlipidemia, and a sedentary lifestyle.During physical examination, vital...
176
Coronary Artery Disease II: Pathophysiology01:26

Coronary Artery Disease II: Pathophysiology

Coronary Artery Disease (CAD) originates from a series of events that impair the function of coronary arteries, the blood vessels responsible for delivering oxygen-rich blood to the heart muscle. The pathophysiology of CAD is closely linked to atherosclerosis, a chronic inflammatory and lipid-driven condition affecting the vascular endothelium.1. Endothelial DamageThe process begins with damage to the vascular endothelium, which serves as a protective barrier between the blood and the vessel...
322
Acute Coronary Syndrome II: Pathophysiology and Clinical Manifestations01:19

Acute Coronary Syndrome II: Pathophysiology and Clinical Manifestations

The pathophysiology of Acute Coronary Syndrome [ACD] involves several key processes:The main underlying cause of ACD is atherosclerosis, a chronic inflammatory disease characterized by the buildup of lipid-laden plaques within the coronary arteries.As the atherosclerotic plaque grows in the coronary artery, it may become unstable due to the formation of a lipid-rich core and a thin fibrous cap. Inflammatory cells within the plaque, such as macrophages, secrete enzymes that degrade the...
318
Acute Coronary Syndrome I: Introduction01:30

Acute Coronary Syndrome I: Introduction

Acute Coronary Syndrome (ACS) encompasses a spectrum of heart conditions caused by sudden obstruction of coronary arteries, typically resulting from the rupture of an atherosclerotic plaque and subsequent thrombus (blood clot) formation. This obstruction can lead to partial or complete blockage of blood flow, causing varying degrees of myocardial ischemia or infarction.ACS includes the following clinical entities:Unstable Angina (UA)Non-ST-Elevation Myocardial Infarction (NSTEMI)ST-Elevation...
665
Cardiomyopathy III: Hypertrophic Cardiomyopathy01:29

Cardiomyopathy III: Hypertrophic Cardiomyopathy

Hypertrophic cardiomyopathy, or HCM, is an autosomal dominant genetic disorder characterized by asymmetric left ventricular hypertrophy without ventricular dilation. It is more common in men and is typically diagnosed in young, athletic adults.EtiologyHCM is primarily genetic and is caused by mutations in genes encoding sarcomeric proteins. Researchers have identified over 1400 mutations across at least 11 different genes. Among these, the most frequently occurring mutations are found in the...
329