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Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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Updated: Jan 3, 2026

A Melanoma Patient-Derived Xenograft Model

A Melanoma Patient-Derived Xenograft Model

Published on: May 20, 2019

A Versatile ES Cell-Based Melanoma Mouse Modeling Platform.

Ilah Bok^1,2, Olga Vera¹, Xiaonan Xu¹

¹Department of Molecular Oncology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, Florida.

Cancer Research

|November 21, 2019

Summary

We developed a rapid embryonic stem cell-based genetically engineered mouse model (GEMM) platform for melanoma research. This versatile platform accelerates the study of melanoma biology using advanced genetic tools.

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Last Updated: Jan 3, 2026

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Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

Published on: June 7, 2019

Area of Science:

Cancer Research
Genetics
Mouse Models

Background:

Generating genetically engineered mouse models (GEMMs) for cancer research is often slow and complex.
Existing methods hinder the rapid study of melanoma using GEMMs.

Purpose of the Study:

To develop and validate a rapid, versatile embryonic stem cell (ESC)-based GEMM platform for melanoma modeling.
To enable high-throughput studies of melanoma biology using advanced genetic tools.

Main Methods:

Developed an ESC-GEMM platform with 12 clinically relevant genotypes for melanoma.
Utilized CRISPR-Cas9, RNAi, and conditional knockout for gene modulation in melanocytes.
Generated and characterized chimera-derived melanoma cell lines.

Main Results:

The ESC-GEMM platform rapidly recapitulates melanoma phenotypes.
Demonstrated the utility of gene depletion and restoration for melanoma modeling.
Chimera-derived cell lines are effective for in vitro and in vivo studies.

Conclusions:

The ESC-GEMM platform combined with genetic tools enables rapid, high-throughput melanoma research.
This platform facilitates addressing outstanding questions in melanoma biology.
The developed platform offers a versatile resource for cancer research.