Related Experiment Video
Updated: Jan 3, 2026
08:20
In Vitro Assay of Plasmodium-Infected Red Blood Cell Killing by Cytotoxic Lymphocytes
Published on: August 17, 2022
2.5K
Assessing the Role of MIF in Plasmodium spp. Infections Using Ex Vivo Models
1Department of Medicine (RMH), Peter Doherty Institute, University of Melbourne, Melbourne, VIC, Australia. Elizabeth.aitken@unimelb.edu.au.
Methods in Molecular Biology (Clifton, N.J.)
|November 21, 2019
Abstract:
Ex vivo techniques are a valuable tool for the investigation of how immune cells respond to Plasmodium spp. antigen, allowing examination of various aspects of the immune response under controlled conditions. Here we describe how to isolate peripheral blood mononuclear cells (PBMC) from donors and coculture them with purified P. falciparum-infected red blood cells (iRBC) to investigate the role of MIF during Plasmodium spp. infection.
