Related Experiment Video
Updated: Jan 3, 2026
Author Spotlight: Plant Primary Organs Profiling Using 13C6-Glucose Labeling and LC-MS
Published on: March 22, 2024
A Method for Extraction and LC-MS-Based Identification of Carotenoid-Derived Dialdehydes in Plants
Jianing Mi1, Kun-Peng Jia1, Aparna Balakrishna1
1The BioActives Lab, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Kingdom of Saudi Arabia.
Abstract:
We developed a chemical derivatization based ultra-high performance liquid chromatography-hybrid quadrupole-Orbitrap mass spectrometer (UHPLC-Q-Orbitrap MS) analytical method to identify low-abundant and instable carotenoid-derived dialdehydes (DIALs, diapocarotenoids) from plants. Application of this method enhances the MS response signal of DIALs, enabling the detection of diapocarotenoids, which is crucial for understanding the function of these compounds and for elucidating the carotenoid oxidative metabolic pathway in plants.
